Enzyme responsive titanium substrates with antibacterial property and osteo/angio-genic differentiation potentials

[Display omitted] •Titanium substrates are fabricated with anodic oxidation followed by drug loading and LBL coating.•The DFO release is associated with the degradation of multilayers triggered by hyaluronidase.•The titanium substrates display good antibacterial and pro-osteo/angiogenic properties....

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Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2020-01, Vol.185, p.110592-110592, Article 110592
Main Authors: Yu, Yonglin, Ran, Qichun, Shen, Xinkun, Zheng, Hong, Cai, Kaiyong
Format: Article
Language:English
Subjects:
Antibacterial
Deferoxamine
Layer-by-layer
osteo/angio-genic differentiation
Titania nanotubes
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Summary:[Display omitted] •Titanium substrates are fabricated with anodic oxidation followed by drug loading and LBL coating.•The DFO release is associated with the degradation of multilayers triggered by hyaluronidase.•The titanium substrates display good antibacterial and pro-osteo/angiogenic properties. After implantation into a host, titanium (Ti) orthopaedic materials are facing two major clinical challenges: bacterial infection and aseptic loosening, which directly determine the long-term survival of the implant. To endow Ti implant with self-defensive antibacterial properties and desirable osteo/angio-genic differentiation potentials, hyaluronic acid (HA)-gentamicin (Gen) conjugates (HA-Gen) and chitosan (Chi) polyelectrolyte multilayers were constructed on deferoxamine (DFO) loaded titania nanotubes (TNT) substrates via layer-by-layer (LBL) assembly technique, termed as TNT/DFO/HA-Gen. The HA-Gen conjugate was characterized by Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (1H NMR). The physicochemical properties of the substrates were characterized by field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and contact angle measurements. The on-demand DFO release was associated with the degradation of multilayers triggered by exogenous hyaluronidase, which indicated enzymatic and bacterial responsiveness. The TNT/DFO/HA-Gen substrates displayed effective antifouling and antibacterial properties against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), while were favourable for the adhesion, proliferation and osteo/angio-genic differentiation of mesenchymal stem cells (MSCs). The multifaceted drug-device combination (DDC) strategy showed potential applications in orthopaedic fields.
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2019.110592