Effect of methyl jasmonate on carotenoids biosynthesis in germinated maize kernels

• Published in: Food chemistry 2020-03, Vol.307, p.125525-125525, Article 125525
• Main Authors: Luo, Hao, He, Weiwei, Li, Dajing, Bao, Yihong, Riaz, Asad, Xiao, Yadong, Song, Jiangfeng, Liu, Chunquan
• Format: Article
• Language: English
• Subjects: Acetates - pharmacology; Carotenoids; Carotenoids - analysis; Carotenoids - metabolism; Chromatography, High Pressure Liquid; Cyclopentanes - pharmacology; Free Radical Scavengers - chemistry; Gene expression; Gene Expression Regulation, Plant - drug effects; Germinated maize kernels; Germination; Lutein - analysis; Methyl jasmonate; Oxylipins - pharmacology; Plant Proteins - genetics; Plant Proteins - metabolism; Proline - analysis; Zea mays - growth & development; Zea mays - metabolism
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2019.125525

•Appropriate methyl jasmonate dose induced enrichment of carotenoids in germinated maize kernels.•Methyl jasmonate could increase germination percentage and radical scavenging activity.•Salicyl hydroxamic acid treatment reduced carotenoids accumulation in germinated maize kernels.•The transcript levels of most carotenoid biosynthetic genes increased by methyl jasmonate. This study investigated effects of different concentrations of methyl jasmonate (MeJA) on carotenoids accumulation, radical scavenging activity and proline content in germinated maize kernels. MeJA treatment promoted carotenoids accumulation, radical scavenging activity and proline accumulation, while salicyl hydroxamic acid (SHAM) reduced carotenoids accumulation. There was a significant increase of 42.5% in lutein content when treated with 0.5 μM MeJA. Furthermore, the transcriptional expression of seven carotenogenic genes were explored by MeJA and SHAM. The results showed that 0.5 μM MeJA significantly increased the gene expression levels of PSY, PDS, ZDS, LCYB, LCYE, BCH1, CYP97C, and their transcript levels, which were strongly associated with carotenoids content. Treatment of MeJA affected the carotenoids biosynthesis gene and led to the accumulation of carotenoids. These new findings would help to develop innovative approach for enrichment of lutein in germinated maize kernels for further development of functional food materials.