Anti‐inflammatory and anti‐resorptive efficacy of adrenergic blockers on late replanted rat incisors

• Published in: Dental traumatology 2020-06, Vol.36 (3), p.253-263
• Main Authors: Matos, Felipe de Souza, Prado, Renata Falchete, Khoury, Rayana Duarte, Oliveira, Luciane Dias, Valera, Marcia Carneiro, Tomson, Phillip Leo, Carvalho, Cláudio Antonio Talge
• Format: Article
• Language: English
• Subjects: adrenergic blockers; Alveoli; Biocompatibility; Calcium hydroxide; Carboxymethylcellulose; Cell survival; Cytotoxicity; Dentistry; Endodontics; Fibroblasts; Gels; Incisors; Inflammation; Lipopolysaccharides; Osteoclastogenesis; Osteoclasts; Periodontal ligament; Phentolamine; Propranolol; Root canals; Root resorption; Surgery; tooth avulsion; tooth replantation
• ISSN: 1600-4469; 1600-9657
• DOI: 10.1111/edt.12523

Background/Aim In addition to their anti‐inflammatory and anti‐osteoclastogenesis properties, adrenergic blockers may also have promising anti‐resorptive effects that can prevent root resorption when teeth are replanted because of avulsion. The aim of this study was to investigate the effects of phentolamine (Ph) and propranolol (Pr) in gels on the repair process of late replanted rat incisors. A further aim was to evaluate the biocompatibility of both drugs to human periodontal ligament fibroblasts (HPDLFs). Materials and methods Forty‐eight maxillary right incisors were extracted from male Wistar rats, stored in paper napkins for 60 minutes, and randomly allocated to one of eight groups (n = 6). The root canal, root surface, and alveolus were treated with 0.75 µg/mL Ph gel (Ph0.75), 10 µg/mL Ph gel (Ph10), 100 µg/mL Ph gel (Ph100), 2.5 µg/mL Pr gel (Pr2.5), 10 µg/mL Pr gel (Pr10), 100 µg/mL Pr gel (Pr100), or sodium carboxymethylcellulose gel (CMC) before replantation. In the control group (CH), only the root canal was treated with calcium hydroxide paste. Thirty days following surgery, the animals were euthanized, and the right hemimaxilla was removed to perform micro‐CT and histomorphometric analysis to determine osteoclastic activity. Ethanolic solutions of Ph10 and Pr10 were selected based on the in vivo study, and the viability of HPDLFs stimulated with lipopolysaccharide was determined by MTT assays. Results The micro‐CT and histomorphometric analysis revealed no significant differences among the treatments (P > .05). The presence of active osteoclasts was significantly decreased in the Ph10 and Pr10 groups (P  .05). Conclusions Ph10 and Pr10 significantly decreased osteoclastogenesis in delayed replanted rat teeth and were not cytotoxic toward HPDLFs.