Normal and cancer cells response on the thin films based on chitosan and tannic acid

• Published in: Toxicology in vitro 2020-02, Vol.62, p.104688-104688, Article 104688
• Main Authors: Kaczmarek, Beata, Miłek, Oliwia, Nadolna, Kinga, Owczarek, Agata, Kleszczyński, Konrad, Osyczka, Anna M.
• Format: Article
• Language: English
• Subjects: Biotechnology; Cell culture; Cell lines; Cell response; Cell viability; Chitosan; In vitro methods and tests; Surface roughness; Tannic acid; Thin films; Tissue engineering
• ISSN: 0887-2333; 1879-3177
• DOI: 10.1016/j.tiv.2019.104688

A novel aspect of tissue engineering is the material selective influence on the different cell types. The cell viability is a parameter which determine the cell ability to proliferate in the contact with material. In the experimental study the thin films based on chitosan and tannic acid mixture in ratio 80/20, 50/50 and 20/80 were tested. The surface roughness decreases with increasing tannic acid content. The cell culture was established on the proposed films. In vitro tests were carried out for the different cell lines as MNT-1, SK-MEL-28, Saos-2, HaCaT and BMSC. The result showed the dependence on the material influence on the various cell lines. •Tannic acid content modulate cell growth of different cell lines.•The ratios of chitosan and tannic acid influence the percentage number of metabolically active cells.•20% addition of tannic acid results in the inhibition of MNT-1 cell growth.•Materials composed of chitosan and tannic acid may find applications as cell growth surfaces.