Siderophore-assisted cadmium hyperaccumulation in Bacillus subtilis

Siderophores (Gk iron carriers) are low molecular weight secondary metabolites produced by bacteria, fungi, and plants that have strong binding affinity for iron. Owing to their iron-chelating ability, they are produced mainly when the organism faces iron scarcity. The present study empirically inve...

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Bibliographic Details
Published in:International microbiology 2020-05, Vol.23 (2), p.277-286
Main Authors: Khan, Azmi, Gupta, Adity, Singh, Pratika, Mishra, Arun Kumar, Ranjan, Rajesh Kumar, Srivastava, Amrita
Format: Article
Language:English
Subjects:
Accumulation
Affinity
Applied Microbiology
Atomic absorption spectroscopy
Bacillus subtilis
Biomedical and Life Sciences
Bioremediation
Cadmium
Carbohydrates
Chelation
Eukaryotic Microbiology
Ferric ions
Heavy metals
Intracellular
Ions
Iron
Life Sciences
Lipid peroxidation
Lipids
Low molecular weights
Medical Microbiology
Metabolites
Microbial Ecology
Microbiology
Molecular weight
Original Article
Peroxidation
Secondary metabolites
Siderophores
Spectral analysis
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Summary:Siderophores (Gk iron carriers) are low molecular weight secondary metabolites produced by bacteria, fungi, and plants that have strong binding affinity for iron. Owing to their iron-chelating ability, they are produced mainly when the organism faces iron scarcity. The present study empirically investigated the importance of applying hydroxamate siderophore extracted from Aspergillus nidulans to the cells of Bacillus subtilis for bioremediation of cadmium salt. This investigation deals with siderophore-mediated intracellular Cd accumulation by bacterial cells, growth estimation, biochemical assays like lipid peroxidation, total protein content, carbohydrate content, and iron content estimation. In silico docking and STRING analyses revealed specific interaction between Aspergillus siderophore and receptors present on B . subtilis . Estimation of intracellular Cd by atomic absorption spectroscopy showed more accumulation of Cd ions by B . subtilis in the presence of hydroxamate siderophore. This suggests a possibility of confiscating environmental Cd 2+ by utilizing metal chelation property of siderophores and hence can lead to emerging bioremediation mechanisms for heavy metals. In silico studies support experimental investigation and suggest higher affinity of siderophore for Cd ions as compared with ferric ions.
ISSN:1139-6709
1618-1905
DOI:10.1007/s10123-019-00101-4