Characterization of phenazine-1-carboxylic acid by Klebsiella sp. NP-C49 from the coral environment in Gulf of Kutch, India

Coral-associated microbes from Marine National Park (MNP), Gulf of Kutch (GoK), Gujarat, India, were screened for siderophore production. Maximum siderophore-producing isolate NP-C49 and its compound were identified and characterized. The isolate was identified as Klebsiella sp. through 16S rRNA gen...

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Bibliographic Details
Published in:Archives of microbiology 2020-03, Vol.202 (2), p.351-359
Main Authors: Patel, Neha P., Raju, M., Haldar, Soumya, Chatterjee, Pabitra B.
Format: Article
Language:English
Subjects:
Animals
Anthozoa - microbiology
Anti-Bacterial Agents - pharmacology
Antibiotics
Biochemistry
Biomedical and Life Sciences
Biotechnology
Carboxylic acids
Cell Biology
Ecology
Ferric chloride
Ferric hydroxide
Gene sequencing
Humans
India
Iron chlorides
Klebsiella
Klebsiella - classification
Klebsiella - genetics
Klebsiella - metabolism
Life Sciences
Magnetic Resonance Spectroscopy
Mass Spectrometry
Mass spectroscopy
Microbial Ecology
Microbial Sensitivity Tests
Microbiology
National parks
NMR
Nuclear magnetic resonance
Original Paper
Phenazine
Phenazine-1-carboxylic acid
Phenazines - metabolism
RNA, Ribosomal, 16S - genetics
rRNA 16S
Siderophores - biosynthesis
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Summary:Coral-associated microbes from Marine National Park (MNP), Gulf of Kutch (GoK), Gujarat, India, were screened for siderophore production. Maximum siderophore-producing isolate NP-C49 and its compound were identified and characterized. The isolate was identified as Klebsiella sp. through 16S rRNA genes sequencing (GenBank accession nos. KY412519 and MTCC 25160). Antibiotic susceptibility profile against 20 commercial antibiotics showed its more sensitivity compared to human pathogenic strain, i.e., Klebsiella pneumonia . The compound was identified as phenazine-1-carboxylic acid (PCA) using the multinuclear ID ( 1 H and 13 C) and 2D ( 1 H– 1 H COSY and 1 H– 13 C HETCOR) NMR along with high-resolution mass spectrometry. No significant difference in the bacterial growth in the presence of PCA, FeCl 3 and Fe(OH) 3 indicated involvement of factors other than PCA in bacterial growth. The study first reports the identification and characterization of PCA from Klebsiella sp. both from terrestrial and marine sources.
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-019-01742-9