Investigation of the pharmacodynamic substances in dahuang zhechong pill that inhibit energy metabolism

Dahuang Zhechong pill (DHZCP) is a commonly used traditional Chinese medicine for the treatment of hepatocarcinoma. Previous studies have found that DHZCP can exert anti-hepatocarcinoma effects and reverse drug resistance by inhibiting energy metabolism. The goal of this study was to further explore...

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Bibliographic Details
Published in:Journal of ethnopharmacology 2020-04, Vol.251, p.112332-112332, Article 112332
Main Authors: Ni, Zi Hui, Wu, Li, Cao, Ke Xin, Zhang, Xi Qiong, Wang, Dan Yu, Zeng, Yu Wei, Liang, Lin Lin, Qiu, Xian Dan, Guo, Run Sheng, Cheng, Hai Bo, Chen, Zhi Peng
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
Animals
Cell Line, Tumor
Dahuang zhechong pill
Drugs, Chinese Herbal - chemistry
Drugs, Chinese Herbal - pharmacology
Energy metabolism
Energy Metabolism - drug effects
Hepatocarcinoma
Humans
Male
Pharmacodynamic substance
Phytochemicals - analysis
Phytochemicals - pharmacology
Rats, Sprague-Dawley
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Summary:Dahuang Zhechong pill (DHZCP) is a commonly used traditional Chinese medicine for the treatment of hepatocarcinoma. Previous studies have found that DHZCP can exert anti-hepatocarcinoma effects and reverse drug resistance by inhibiting energy metabolism. The goal of this study was to further explore the pharmacodynamic substances that inhibit energy metabolism. The components of DHZCP absorbed into plasma were identified by UHPLC-Q-TOF-MS/MS. The Swiss and STITCH databases were used for target collection. The DAVID database was used for pathway enrichment analysis. Cytoscape software was used for network construction. The CCK-8 method detected cell viability. Chemiluminescence was used to detect ATP levels. A total of 89 components absorbed into plasma were identified by UHPLC-Q-TOF-MS/MS. Based on this, 24 potential pharmacodynamic substances were selected by network pharmacology. Among them, 11 components such as rhein can significantly inhibit ATP levels. Rhein, emodin, chrysophanol, hypoxanthine, baicalein, baicalin, wogonoside, acteoside, formononetin, isoliquiritigenin, and glycyrrhizic acid were the pharmacodynamic substances responsible for inhibition of energy metabolism of DHZCP. [Display omitted]
ISSN:0378-8741
1872-7573
DOI:10.1016/j.jep.2019.112332