Detection of plasmid-mediated colistin resistance by colistin pre-diffusion and inhibition with EDTA test (CPD-E) in Enterobactereaceae

A phenotypic assay based on colistin pre-diffusion and differential inhibition of Mobile Colistin Resistance (MCR) protein activity by EDTA showed that, of the 92 strains tested, all MCR producers (49) exhibited an increase ≥5 mm in the inhibition zone around the area of pre-diffusion in the presenc...

Full description

Saved in:
Bibliographic Details
Published in:Journal of microbiological methods 2019-12, Vol.167, p.105759-105759, Article 105759
Main Authors: Yauri Condor, Katherine, Gonzales Escalante, Edgar, Di Conza, José, Gutkind, Gabriel
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A phenotypic assay based on colistin pre-diffusion and differential inhibition of Mobile Colistin Resistance (MCR) protein activity by EDTA showed that, of the 92 strains tested, all MCR producers (49) exhibited an increase ≥5 mm in the inhibition zone around the area of pre-diffusion in the presence of EDTA, in comparison with colistin alone. Results suggest that CPD-E may differentiate MCR-producing microorganisms from resistant microorganisms without this marker. •CDP-E can easily differentiate plasmid-mediated colistin resistance from resistance mediated by chromosomal mutations in Enterobacteriaceae.•This new, fast and easy to perform protocol was able to discriminate plasmid-mediated colistin resistance strains.•CDP-E provided a 100% sensitivity and specificity for mcr detection.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2019.105759