The long term effects of intrascrotal low dose and high dose N-acetylcysteine on testis damage in rat model of testicular torsion

• Published in: Journal of pediatric surgery 2020-04, Vol.55 (4), p.672-680
• Main Authors: Acer-Demir, Tuğba, Mammadov, Mirhüseyn, Öcbe, Pınar, Çoruhlu, Asyanur, Coşkun, Dicle, Nazik, Yaren, Tüfekçi, Işıl, Güney, Lütfi Hakan, Hiçsönmez, Akgün
• Format: Article
• Language: English
• Subjects: Ischemia; N-acetylcysteine; Oxidative damage; Oxygen radicals; Reperfusion; Testis torsion
• ISSN: 0022-3468; 1531-5037
• DOI: 10.1016/j.jpedsurg.2019.09.028

During testicular torsion, the testes face oxidative damage owing to ischemia/reperfusion. We studied the long term effects of the intrascrotal administration of N-acetylcysteine (NAC) during detorsion procedure in a rat model of testicular torsion. Twenty-eight rats were divided into 4 groups: (1) Control group: No procedure was done (2): Torsion–detorsion group: Testis torsion applied for 3 h (3): Low Dose Group: After testis torsion–detorsion (for 3 h) 10 mg/kg NAC was given into tunica vaginalis (4): High Dose Group: After testis torsion–detorsion (for 3 h) 100 mg/kg NAC was given into tunica vaginalis. We measured dimensions of the testes and examined pathological findings and Johnsen and Cosantino Scores. For testes height and volume, high dose NAC group had better results than the torsion–detorsion group (p = 0.019, p = 0.049). Testes weight showed no difference (p = 0.204). Sertoli cell number per tubule in the high dose NAC group was statistically different than the torsion–detorsion group (p = 0.017). When NAC was given intrascrotally at a dose of 100 mg/kg, it decreased the loss of testis volume and height, and Sertoli cell number per tubule was similar to the control group. These results suggest that the higher dose intrascrotal NAC administered during detorsion may have a protective effect.