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Propagation phase-contrast micro-computed tomography allows laboratory-based three-dimensional imaging of articular cartilage down to the cellular level
High-resolution non-invasive three-dimensional (3D) imaging of chondrocytes in articular cartilage remains elusive. The aim of this study was to explore whether laboratory micro-computed tomography (micro-CT) permits imaging cells within articular cartilage. Bovine osteochondral plugs were prepared...
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| Published in: | Osteoarthritis and cartilage 2020-01, Vol.28 (1), p.102-111 |
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| container_title | Osteoarthritis and cartilage |
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| creator | Clark, J.N. Garbout, A. Ferreira, S.A. Javaheri, B. Pitsillides, A.A. Rankin, S.M. Jeffers, J.R.T. Hansen, U. |
| description | High-resolution non-invasive three-dimensional (3D) imaging of chondrocytes in articular cartilage remains elusive. The aim of this study was to explore whether laboratory micro-computed tomography (micro-CT) permits imaging cells within articular cartilage.
Bovine osteochondral plugs were prepared four ways: in phosphate-buffered saline (PBS) or 70% ethanol (EtOH), both with or without phosphotungstic acid (PTA) staining. Specimens were imaged with micro-CT following two protocols: 1) absorption contrast (AC) imaging 2) propagation phase-contrast (PPC) imaging. All samples were scanned in liquid. The contrast to noise ratio (C/N) of cellular features quantified scan quality and were statistically analysed. Cellular features resolved by micro-CT were validated by standard histology.
The highest quality images were obtained using propagation phase-contrast imaging and PTA-staining in 70% EtOH. Cellular features were also visualised when stained in PBS and unstained in EtOH. Under all conditions PPC resulted in greater contrast than AC (p |
| doi_str_mv | 10.1016/j.joca.2019.10.007 |
| format | article |
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Bovine osteochondral plugs were prepared four ways: in phosphate-buffered saline (PBS) or 70% ethanol (EtOH), both with or without phosphotungstic acid (PTA) staining. Specimens were imaged with micro-CT following two protocols: 1) absorption contrast (AC) imaging 2) propagation phase-contrast (PPC) imaging. All samples were scanned in liquid. The contrast to noise ratio (C/N) of cellular features quantified scan quality and were statistically analysed. Cellular features resolved by micro-CT were validated by standard histology.
The highest quality images were obtained using propagation phase-contrast imaging and PTA-staining in 70% EtOH. Cellular features were also visualised when stained in PBS and unstained in EtOH. Under all conditions PPC resulted in greater contrast than AC (p < 0.0001 to p = 0.038). Simultaneous imaging of cartilage and subchondral bone did not impede image quality. Corresponding features were located in both histology and micro-CT and followed the same distribution with similar density and roundness values.
Three-dimensional visualisation and quantification of the chondrocyte population within articular cartilage can be achieved across a field of view of several millimetres using laboratory-based micro-CT. The ability to map chondrocytes in 3D opens possibilities for research in fields from skeletal development through to medical device design and treatment of cartilage degeneration.</description><identifier>ISSN: 1063-4584</identifier><identifier>EISSN: 1522-9653</identifier><identifier>DOI: 10.1016/j.joca.2019.10.007</identifier><identifier>PMID: 31678663</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Articular cartilage ; Cartilage, Articular - cytology ; Cartilage, Articular - ultrastructure ; Cattle ; Chondrocyte ; Chondrocytes - ultrastructure ; Contrast Media ; Imaging ; Imaging, Three-Dimensional - methods ; Micro-CT ; Microscopy, Phase-Contrast - methods ; Osteoarthritis ; X-Ray Microtomography - methods</subject><ispartof>Osteoarthritis and cartilage, 2020-01, Vol.28 (1), p.102-111</ispartof><rights>2019 Osteoarthritis Research Society International</rights><rights>Copyright © 2019 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c400t-e33beaba1ac0f140bc274f7514b268f6b5fc55422a335e5fb7acf5a4a1fd0c9c3</citedby><cites>FETCH-LOGICAL-c400t-e33beaba1ac0f140bc274f7514b268f6b5fc55422a335e5fb7acf5a4a1fd0c9c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31678663$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Clark, J.N.</creatorcontrib><creatorcontrib>Garbout, A.</creatorcontrib><creatorcontrib>Ferreira, S.A.</creatorcontrib><creatorcontrib>Javaheri, B.</creatorcontrib><creatorcontrib>Pitsillides, A.A.</creatorcontrib><creatorcontrib>Rankin, S.M.</creatorcontrib><creatorcontrib>Jeffers, J.R.T.</creatorcontrib><creatorcontrib>Hansen, U.</creatorcontrib><title>Propagation phase-contrast micro-computed tomography allows laboratory-based three-dimensional imaging of articular cartilage down to the cellular level</title><title>Osteoarthritis and cartilage</title><addtitle>Osteoarthritis Cartilage</addtitle><description>High-resolution non-invasive three-dimensional (3D) imaging of chondrocytes in articular cartilage remains elusive. The aim of this study was to explore whether laboratory micro-computed tomography (micro-CT) permits imaging cells within articular cartilage.
Bovine osteochondral plugs were prepared four ways: in phosphate-buffered saline (PBS) or 70% ethanol (EtOH), both with or without phosphotungstic acid (PTA) staining. Specimens were imaged with micro-CT following two protocols: 1) absorption contrast (AC) imaging 2) propagation phase-contrast (PPC) imaging. All samples were scanned in liquid. The contrast to noise ratio (C/N) of cellular features quantified scan quality and were statistically analysed. Cellular features resolved by micro-CT were validated by standard histology.
The highest quality images were obtained using propagation phase-contrast imaging and PTA-staining in 70% EtOH. Cellular features were also visualised when stained in PBS and unstained in EtOH. Under all conditions PPC resulted in greater contrast than AC (p < 0.0001 to p = 0.038). Simultaneous imaging of cartilage and subchondral bone did not impede image quality. Corresponding features were located in both histology and micro-CT and followed the same distribution with similar density and roundness values.
Three-dimensional visualisation and quantification of the chondrocyte population within articular cartilage can be achieved across a field of view of several millimetres using laboratory-based micro-CT. The ability to map chondrocytes in 3D opens possibilities for research in fields from skeletal development through to medical device design and treatment of cartilage degeneration.</description><subject>Animals</subject><subject>Articular cartilage</subject><subject>Cartilage, Articular - cytology</subject><subject>Cartilage, Articular - ultrastructure</subject><subject>Cattle</subject><subject>Chondrocyte</subject><subject>Chondrocytes - ultrastructure</subject><subject>Contrast Media</subject><subject>Imaging</subject><subject>Imaging, Three-Dimensional - methods</subject><subject>Micro-CT</subject><subject>Microscopy, Phase-Contrast - methods</subject><subject>Osteoarthritis</subject><subject>X-Ray Microtomography - methods</subject><issn>1063-4584</issn><issn>1522-9653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9Uctu1DAUtRAVLVN-gAXykk0GP-JkRmKDqvKQKsGiXVs3znXGIycOttNq_oTPxWEKS1b34XOOfM8h5C1nW8548-G4PQYDW8H4viy2jLUvyBVXQlT7RsmXpWeNrGq1qy_J65SOjDHJOXtFLiVv2l3TyCvy60cMMwyQXZjofICElQlTjpAyHZ2JoYzjvGTsaQ5jGCLMhxMF78NToh66ECGHeKq6wiyQQ0SsejfilIogeOpGGNw00GApxOzM4iFSs7YeBqR9eJqKcCEiNej9n2ePj-ivyYUFn_DNc92Qh8-39zdfq7vvX77dfLqrTM1YrlDKDqEDDoZZXrPOiLa2reJ1J5qdbTpljVK1ECClQmW7FoxVUAO3PTN7Izfk_Vl3juHnginr0aX1KzBhWJIWxbK9aHjxbkPEGVpsSSmi1XMs98WT5kyvieijXhPRayLrriRSSO-e9ZduxP4f5W8EBfDxDMBy5aPDqJNxOBnsXUSTdR_c__R_A1X5of0</recordid><startdate>202001</startdate><enddate>202001</enddate><creator>Clark, J.N.</creator><creator>Garbout, A.</creator><creator>Ferreira, S.A.</creator><creator>Javaheri, B.</creator><creator>Pitsillides, A.A.</creator><creator>Rankin, S.M.</creator><creator>Jeffers, J.R.T.</creator><creator>Hansen, U.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202001</creationdate><title>Propagation phase-contrast micro-computed tomography allows laboratory-based three-dimensional imaging of articular cartilage down to the cellular level</title><author>Clark, J.N. ; Garbout, A. ; Ferreira, S.A. ; Javaheri, B. ; Pitsillides, A.A. ; Rankin, S.M. ; Jeffers, J.R.T. ; Hansen, U.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-e33beaba1ac0f140bc274f7514b268f6b5fc55422a335e5fb7acf5a4a1fd0c9c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Articular cartilage</topic><topic>Cartilage, Articular - cytology</topic><topic>Cartilage, Articular - ultrastructure</topic><topic>Cattle</topic><topic>Chondrocyte</topic><topic>Chondrocytes - ultrastructure</topic><topic>Contrast Media</topic><topic>Imaging</topic><topic>Imaging, Three-Dimensional - methods</topic><topic>Micro-CT</topic><topic>Microscopy, Phase-Contrast - methods</topic><topic>Osteoarthritis</topic><topic>X-Ray Microtomography - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Clark, J.N.</creatorcontrib><creatorcontrib>Garbout, A.</creatorcontrib><creatorcontrib>Ferreira, S.A.</creatorcontrib><creatorcontrib>Javaheri, B.</creatorcontrib><creatorcontrib>Pitsillides, A.A.</creatorcontrib><creatorcontrib>Rankin, S.M.</creatorcontrib><creatorcontrib>Jeffers, J.R.T.</creatorcontrib><creatorcontrib>Hansen, U.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Osteoarthritis and cartilage</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Clark, J.N.</au><au>Garbout, A.</au><au>Ferreira, S.A.</au><au>Javaheri, B.</au><au>Pitsillides, A.A.</au><au>Rankin, S.M.</au><au>Jeffers, J.R.T.</au><au>Hansen, U.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Propagation phase-contrast micro-computed tomography allows laboratory-based three-dimensional imaging of articular cartilage down to the cellular level</atitle><jtitle>Osteoarthritis and cartilage</jtitle><addtitle>Osteoarthritis Cartilage</addtitle><date>2020-01</date><risdate>2020</risdate><volume>28</volume><issue>1</issue><spage>102</spage><epage>111</epage><pages>102-111</pages><issn>1063-4584</issn><eissn>1522-9653</eissn><abstract>High-resolution non-invasive three-dimensional (3D) imaging of chondrocytes in articular cartilage remains elusive. The aim of this study was to explore whether laboratory micro-computed tomography (micro-CT) permits imaging cells within articular cartilage.
Bovine osteochondral plugs were prepared four ways: in phosphate-buffered saline (PBS) or 70% ethanol (EtOH), both with or without phosphotungstic acid (PTA) staining. Specimens were imaged with micro-CT following two protocols: 1) absorption contrast (AC) imaging 2) propagation phase-contrast (PPC) imaging. All samples were scanned in liquid. The contrast to noise ratio (C/N) of cellular features quantified scan quality and were statistically analysed. Cellular features resolved by micro-CT were validated by standard histology.
The highest quality images were obtained using propagation phase-contrast imaging and PTA-staining in 70% EtOH. Cellular features were also visualised when stained in PBS and unstained in EtOH. Under all conditions PPC resulted in greater contrast than AC (p < 0.0001 to p = 0.038). Simultaneous imaging of cartilage and subchondral bone did not impede image quality. Corresponding features were located in both histology and micro-CT and followed the same distribution with similar density and roundness values.
Three-dimensional visualisation and quantification of the chondrocyte population within articular cartilage can be achieved across a field of view of several millimetres using laboratory-based micro-CT. The ability to map chondrocytes in 3D opens possibilities for research in fields from skeletal development through to medical device design and treatment of cartilage degeneration.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>31678663</pmid><doi>10.1016/j.joca.2019.10.007</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Articular cartilage Cartilage, Articular - cytology Cartilage, Articular - ultrastructure Cattle Chondrocyte Chondrocytes - ultrastructure Contrast Media Imaging Imaging, Three-Dimensional - methods Micro-CT Microscopy, Phase-Contrast - methods Osteoarthritis X-Ray Microtomography - methods |
| title | Propagation phase-contrast micro-computed tomography allows laboratory-based three-dimensional imaging of articular cartilage down to the cellular level |
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