Molecular Bases of DNA Packaging in Bacteria Revealed by All-Atom Molecular Dynamics Simulations: The Case of Histone-Like Proteins in Borrelia burgdorferi

DNA compaction is essential to ensure the packaging of the genetic material in living cells and also plays a key role in the epigenetic regulation of gene expression. In both humans and bacteria, DNA packaging is achieved by specific well-conserved proteins. Here, by means of all-atom molecular dyna...

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Bibliographic Details
Published in:The journal of physical chemistry letters 2019-11, Vol.10 (22), p.7200-7207
Main Authors: Hognon, Cécilia, Garaude, Simon, Timmins, Joanna, Chipot, Christophe, Dehez, François, Monari, Antonio
Format: Article
Language:English
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Summary:DNA compaction is essential to ensure the packaging of the genetic material in living cells and also plays a key role in the epigenetic regulation of gene expression. In both humans and bacteria, DNA packaging is achieved by specific well-conserved proteins. Here, by means of all-atom molecular dynamics simulations, including the determination of relevant free-energy profiles, we rationalize the molecular bases for this remarkable process in bacteria, illustrating the crucial role played by positively charged amino acids of a small histone-like protein. We also present compelling evidence that this histone-like protein alone can induce strong bending of a DNA duplex around its core domain, a process that requires overcoming a major free-energy barrier.
ISSN:1948-7185
1948-7185
DOI:10.1021/acs.jpclett.9b02978