Total flavonoids of Epimedium ameliorates testicular damage in streptozotocin‐induced diabetic rats by suppressing inflammation and oxidative stress

• Published in: Environmental toxicology 2020-02, Vol.35 (2), p.268-276
• Main Authors: Cheng, Yin, Yang, Zhangliang, Shi, Jie, Yang, Junjie, Zhao, Jinguo, He, Yinghao, Qi, Minyou
• Format: Article
• Language: English
• Subjects: Animal tissues; Animals; Biological stress; Biomarkers; Biomarkers - blood; Blood; Damage; Diabetes; Diabetes mellitus; Diabetes Mellitus, Experimental - blood; Diabetes Mellitus, Experimental - immunology; Diabetes Mellitus, Experimental - metabolism; Epididymis; Epididymis - drug effects; Epididymis - immunology; Epididymis - pathology; Epimedium; Epimedium - chemistry; Flavonoids; Flavonoids - isolation & purification; Flavonoids - pharmacology; Glucose; Growth factors; Histopathology; Immunohistochemistry; Indicators; Inflammation; Interleukins; Male; Monocyte chemoattractant protein; Monocytes; Oxidative stress; Oxidative Stress - drug effects; Oxidative Stress - immunology; Proteins; Random Allocation; Rats; Rats, Sprague-Dawley; Streptozocin; Testes; testis; Testis - drug effects; Testis - immunology; Testis - pathology; Testosterone; total flavonoids of Epimedium
• ISSN: 1520-4081; 1522-7278
• DOI: 10.1002/tox.22864

Testicular damage is the anomaly that will often accompany diabetes mellitus. Thus, this study aimed to investigate the role that total flavonoids of Epimedium (TFE) played against streptozotocin (STZ)‐induced diabetic testicular dysfunction and to elucidate the mechanism of action. The diabetic rat model was induced by vein injection of STZ in healthy rats. Thirty male healthy Spraque‐Dawley rats were randomly divided into following groups: the control group, the diabetic group, and the diabetic + TFE group. Gastrointestinal administration begins at fifth week of TFE for 6 weeks. After TFE administration, all animals were euthanized. Testicular tissue samples and blood samples of rats were collected for histopathological examination and for determination of levels of various biomarkers including blood glucose, testosterone, testicular enzymes, and oxidative stress indicators. All testes were weighted to calculate the testicular organ index. Hematoxylin‐eosin staining was used for observing the testis and epididymis pathological changes. Protein expression (monocyte chemoattractant protein‐1, transforming growth factor‐beta‐1, interleukin‐6, and 3‐beta‐hydroxysteroid dehydrogenase) was detected by immunohistochemistry and western blot techniques. There was a significant difference in the changes between the diabetes group and the control group. As a result of treat with TFE, the blood glucose decreased but there was no significant difference, and other indicators showed significant improvement. TFE may protect against STZ‐induced testicular injury by suppressing inflammation and oxidative stress.