Ortholinea scatophagi (Myxosporea: Ortholineidae), a novel myxosporean infecting the spotted scat, Scatophagus argus (Linnaeus 1766) from southwest coast of India

• Published in: Parasitology international 2020-04, Vol.75, p.102020-102020, Article 102020
• Main Authors: Chandran, Archana, Zacharia, P.U., Sanil, N.K.
• Format: Article
• Language: English
• Subjects: Extra-sutural ridges; Molecular phylogeny; Myxosporeans; Ortholinea scatophagi; Scatophagus argus; Spores; Urinary system
• ISSN: 1383-5769; 1873-0329
• DOI: 10.1016/j.parint.2019.102020

A new species of myxosporean, Ortholinea scatophagi n. sp. infecting the urinary bladder of the spotted scat, Scatophagus argus (Linnaeus 1766) is described. O. scatophagi n. sp. is characterized by spherical myxospores with a slightly flattened anterior end and equal spore valves with extra sutural ridges on its surface; measured 7.34 ± 0.67 μm in length, 6.90 ± 0.71 μm in width and 6.48 ± 0.37 μm in thickness. Two polar capsules, equal, spherical to oval in shape, arranged diametrically opposite and measured 2.59 ± 0.42 μm in length and 2.24 ± 0.35 μm in width. Polar filaments, 21.84 ± 2.86 μm long, with four to five coils. Scanning electron microscopy revealed the presence of extra sutural ridges on spore surface. Pansporoblasts spherical to irregular in shape, measured 31.08 ± 2.67 μm in length and 13.88 ± 5.40 μm in width; Monosporic, disporic and polysporic plasmodial stages were observed; plasmodia spherical or irregular in shape with granular cytoplasm containing refractile granules. The species was compared with 23 existing nominal species of Ortholinea, based on morphology and morphometry. Molecular analysis resulted in a 1773 bp long SSU rDNA sequence (GenBank accession number MN 310514). In phylogenetic analyses the present parasite clustered with other members of Ortholinea, under the freshwater urinary clade. Considering the morphologic, morphometric and molecular differences with previously described species of Ortholinea, and differences in host and geographic locations, the present species is treated as new and the name Ortholinea scatophagi n. sp.is proposed. [Display omitted] •Describes Ortholinea scatophagi n. sp., a new species of myxosporean infecting the urinary bladder of the spotted scat.•Spores and developmental stages of the parasite are described in detail.•Scanning electron microscopy revealed the presence of extra sutural ridges on spore surface.•Morphological, molecular and phylogenetic analysis strongly supports the validity of the new species.