Highly specific and sensitive detection of microRNAs by tandem signal amplification based on duplex-specific nuclease and strand displacement

Based on the full understanding of the hydrolysis patterns of duplex-specific nuclease (DSN) against the probe DNAs in DNA/microRNA heteroduplexes, a simple and generic platform for highly specific and sensitive detection of microRNAs was developed by seamlessly integrating DSN-assisted target recyc...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2019-12, Vol.55 (94), p.1421-14213
Main Authors: Liu, Qing, Kang, Peng-Jian, Chen, Zeng-Ping, Shi, Cai-Xia, Chen, Yao, Yu, Ru-Qin
Format: Article
Language:English
Subjects:
Amplification
Cell Line, Tumor
DNA Probes - chemistry
HeLa Cells
Humans
Hydrolysis
MicroRNAs
MicroRNAs - genetics
Nuclease
Nucleic Acid Amplification Techniques
Reverse Transcriptase Polymerase Chain Reaction
Ribonucleic acid
RNA
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Summary:Based on the full understanding of the hydrolysis patterns of duplex-specific nuclease (DSN) against the probe DNAs in DNA/microRNA heteroduplexes, a simple and generic platform for highly specific and sensitive detection of microRNAs was developed by seamlessly integrating DSN-assisted target recycling amplification and strand displacement amplification in tandem. A platform for microRNA detection was developed based on understanding the hydrolysis patterns of duplex-specific nuclease against probe DNAs.
ISSN:1359-7345
1364-548X
DOI:10.1039/c9cc06790f