2-Hydroxymelatonin mitigates cadmium stress in cucumis sativus seedlings: Modulation of antioxidant enzymes and polyamines

• Published in: Chemosphere (Oxford) 2020-03, Vol.243, p.125308-125308, Article 125308
• Main Authors: Shah, Anis Ali, Ahmed, Shakil, Ali, Aamir, Yasin, Nasim Ahmad
• Format: Article
• Language: English
• Subjects: Antioxidants - metabolism; Cadmium; Cadmium - metabolism; Cadmium - toxicity; Cucumis sativus; Cucumis sativus - drug effects; Cucumis sativus - enzymology; Cucumis sativus - metabolism; Melatonin - analogs & derivatives; Melatonin - metabolism; Melatonin - pharmacology; Phytotoxic; Polyamines - metabolism; Putrescine; Seedlings - drug effects; Seedlings - enzymology; Seedlings - metabolism; Seeds - metabolism; Spermidine; Spermine
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2019.125308

Cadmium level is continuously increasing in agricultural soils mainly due to anthropogenic activities. Cadmium is one of the most phytotoxic metals in the soils. The present study investigates the possible role of 2-hydroxymelatonin (2-OHMT) in assuagement of Cd-toxicity in cucumber (Cucumis sativus L.) plants. 2-OHMT is an important metabolite produced through interaction of melatonin with oxygenated compounds. Cadmium stress decreased the activity of antioxidant enzymes and polyamines. However, exogenously applied 2-OHMT enhanced plant growth attributes including photosynthetic rate, intercellular CO2 concentration, stomatal conductance and transpiration rate in treated plants. In addition, 2-OHMT induced enhancement of the activity of PAs biosynthesizing enzymes (putrescine, spermidine and spermine) in conjunction with reduction in activity of polyamine oxidase (PAO). 2-OHMT mitigated Cd stress through up-regulation in expression of stress related CS-ERS gene along with the amplified activity of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) in treated seedlings. The improved activity of antioxidant scavengers played central role in reduction of hydrogen peroxide (H2O2), electrolyte leakage (EL) and malondialdehyde (MDA) in plants under Cd stress. Recent findings also advocate the positive correlation between PAs and ethylene, as both possess common precursor. The current study reveals that priming seeds with 2-OHMT reduces Cd-toxicity and makes it possible to cultivate cucumber in Cd-contaminated areas. Future experiments will perhaps help in elucidation of 2-OHMT intervened stress mitigation procedure in C. sativus crop. Furthermore, research with reference to potential of 2-OHMT for stress alleviation in other horticultural and agronomic crops will assist in enhancement of crop productivity. •Melatonin regulates physiological and metabolic activities of plants under stress conditions.•We found that cadmium stress affected overall growth of Cucumis sativus seedlings.•2-Hydroxymelatonin enhanced photosynthetic rate and transpiration rate in treated plants.•Modulation in activity of polyamines and antioxidants are important mechanisms for cadmium tolerance.