Cathepsin B Turning Bioluminescence “On” for Tumor Imaging

Cathepsin B (CTSB) is a lysosomal protease, and several human cancers are reported highly expressing CTSB. Many optical methods have been developed for CTSB detection but not a bioluminescence (BL) probe. Herein, a CTSB-specific bioluminescence probe Val-Cit-AL was rationally designed for selectivel...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2019-12, Vol.91 (23), p.14834-14837
Main Authors: Ni, Yanhan, Hai, Zijuan, Zhang, Tong, Wang, Yanfang, Yang, Yanyun, Zhang, Shusheng, Liang, Gaolin
Format: Article
Language:English
Subjects:
Animal models
Biological activity
Bioluminescence
Cathepsin B
Chemistry
Optics
Tumors
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Summary:Cathepsin B (CTSB) is a lysosomal protease, and several human cancers are reported highly expressing CTSB. Many optical methods have been developed for CTSB detection but not a bioluminescence (BL) probe. Herein, a CTSB-specific bioluminescence probe Val-Cit-AL was rationally designed for selectively sensing CTSB activity in vitro with a 67-fold “Turn-On” of BL intensity and an excellent limit of detection. Inhibitory experiments indicated that Val-Cit-AL is capable of sensing CTSB activity in living cells and tumors. We anticipate that Val-Cit-AL might be applied to diagnose CTSB-related diseases in rodent models or evaluate CTSB roles in more biological processes in the near future.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.9b04254