Vitamin D Measurement, the Debates Continue, New Analytes Have Emerged, Developments Have Variable Outcomes

The demand for measurement of vitamin D metabolites for clinical diagnosis and to advance our understanding of the role of vitamin D in human health has significantly increased in the last decade. New developments in technologies employed have enabled the separation and quantification of additional...

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Bibliographic Details
Published in:Calcified tissue international 2020, Vol.106 (1), p.3-13
Main Authors: Fraser, William D., Tang, Jonathan C. Y., Dutton, John J., Schoenmakers, Inez
Format: Article
Language:English
Subjects:
25-Hydroxyvitamin D
Biochemistry
Biomedical and Life Sciences
Cell Biology
Diagnosis
Endocrinology
Life Sciences
Metabolites
Orthopedics
Quality assurance
Quality control
Review
Vitamin D
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Summary:The demand for measurement of vitamin D metabolites for clinical diagnosis and to advance our understanding of the role of vitamin D in human health has significantly increased in the last decade. New developments in technologies employed have enabled the separation and quantification of additional metabolites and interferences. Also, developments of immunoassays have changed the landscape. Programmes and materials for assay standardisation, harmonisation and the expansion of the vitamin D external quality assurance scheme (DEQAS) with the provision of target values as measured by a reference measurement procedure have improved standardisation, quality assurance and comparability of measurements. In this article, we describe developments in the measurement of the commonly analysed vitamin D metabolites in clinical and research practice. We describe current analytical approaches, discuss differences between assays, their origin, and how these may be influenced by physiological and experimental conditions. The value of measuring metabolites beyond 25 hydroxyvitamin D (25(OH)D), the marker of vitamin D status, in routine clinical practice is not yet confirmed. Here we provide an overview of the value and application of the measurement of 1,25 dihydroxyvitamin D, 24,25 dihydroxyvitamin D and free 25OHD in the diagnosis of patients with abnormalities in vitamin D metabolism and for research purposes.
ISSN:0171-967X
1432-0827
DOI:10.1007/s00223-019-00620-2