Upstream ORF-Encoded ASDURF Is a Novel Prefoldin-like Subunit of the PAQosome

The PAQosome is an 11-subunit chaperone involved in the biogenesis of several human protein complexes. We show that ASDURF, a recently discovered upstream open reading frame (uORF) in the 5′ UTR of ASNSD1 mRNA, encodes the 12th subunit of the PAQosome. ASDURF displays significant structural homology...

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Bibliographic Details
Published in:Journal of proteome research 2020-01, Vol.19 (1), p.18-27
Main Authors: Cloutier, Philippe, Poitras, Christian, Faubert, Denis, Bouchard, Annie, Blanchette, Mathieu, Gauthier, Marie-Soleil, Coulombe, Benoit
Format: Article
Language:English
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Summary:The PAQosome is an 11-subunit chaperone involved in the biogenesis of several human protein complexes. We show that ASDURF, a recently discovered upstream open reading frame (uORF) in the 5′ UTR of ASNSD1 mRNA, encodes the 12th subunit of the PAQosome. ASDURF displays significant structural homology to β-prefoldins and assembles with the five known subunits of the prefoldin-like module of the PAQosome to form a heterohexameric prefoldin-like complex. A model of the PAQosome prefoldin-like module is presented. The data presented here provide an example of a eukaryotic uORF-encoded polypeptide whose function is not limited to cis-acting translational regulation of downstream coding sequence and highlights the importance of including alternative ORF products in proteomic studies.
ISSN:1535-3893
1535-3907
DOI:10.1021/acs.jproteome.9b00599