Identification of Quinolinols as Activators of TEAD-Dependent Transcription

The transcriptional co-regulators YAP (Yes-associated protein) and TAZ (transcriptional coactivator with PDZ-binding motif) are the vertebrate downstream effectors of the Hippo signaling pathway that controls various physiological and pathological processes. YAP and TAZ pair with the TEAD (TEA domai...

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Published in:ACS chemical biology 2019-12, Vol.14 (12), p.2909-2921
Main Authors: Pobbati, Ajaybabu V, Mejuch, Tom, Chakraborty, Sayan, Karatas, Hacer, Bharath, Sakshibeedu R, Guéret, Stéphanie M, Goy, Pierre-Alexis, Hahne, Gernot, Pahl, Axel, Sievers, Sonja, Guccione, Ernesto, Song, Haiwei, Waldmann, Herbert, Hong, Wanjin
Format: Article
Language:English
Subjects:
Animals
HEK293 Cells
Humans
Hydroxyquinolines - chemistry
Hydroxyquinolines - pharmacology
Mice
Mice, Inbred ICR
Skin - drug effects
Structure-Activity Relationship
Transcription Factors - metabolism
Transcription, Genetic - drug effects
Wound Healing - drug effects
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Summary:The transcriptional co-regulators YAP (Yes-associated protein) and TAZ (transcriptional coactivator with PDZ-binding motif) are the vertebrate downstream effectors of the Hippo signaling pathway that controls various physiological and pathological processes. YAP and TAZ pair with the TEAD (TEA domain) family of transcription factors to initiate transcription. We previously identified a tractable pocket in TEADs, which has been physiologically shown to bind palmitate. Herein, a TEAD–palmitate interaction screen was developed to select small molecules occupying the palmitate-binding pocket (PBP) of TEADs. We show that quinolinols were TEAD-binding compounds that augment YAP/TAZ–TEAD activity, which was verified using TEAD reporter assay, RT-qPCR, and RNA-Seq analyses. Structure–activity relationship investigations uncovered the quinolinol substituents that are necessary for TEAD activation. We reveal a novel mechanism where quinolinols stabilize YAP/TAZ protein levels by occupying the PBP. The enhancement of YAP activity by quinolinols accelerates the in vivo wound closure in a mouse wound-healing model. Although small molecules that occupy the PBP have been shown to inhibit YAP/TAZ–TEAD activity, leveraging PBP to activate TEADs is a novel approach.
ISSN:1554-8929
1554-8937
DOI:10.1021/acschembio.9b00786