Multifunctional Nanoscale Metal–Organic Layers for Ratiometric pH and Oxygen Sensing

As a monolayered version of nanoscale metal–organic frameworks (nMOFs), nanoscale metal–organic layers (nMOLs) represent an emerging class of highly tunable two-dimensional materials for hierarchical functionalization and with facile access to analytes. Here we report the design of the first nMOL-ba...

Full description

Saved in:
Bibliographic Details
Published in:Journal of the American Chemical Society 2019-12, Vol.141 (48), p.18964-18969
Main Authors: Lan, Guangxu, Ni, Kaiyuan, You, Eric, Wang, Maolin, Culbert, August, Jiang, Xiaomin, Lin, Wenbin
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:As a monolayered version of nanoscale metal–organic frameworks (nMOFs), nanoscale metal–organic layers (nMOLs) represent an emerging class of highly tunable two-dimensional materials for hierarchical functionalization and with facile access to analytes. Here we report the design of the first nMOL-based biosensor for ratiometric pH and oxygen sensing in mitochondria. Cationic Hf12-Ru nMOL was solvothermally synthesized by laterally connecting Hf12 secondary building units (SBUs) with oxygen-sensitive Ru­(bpy)3 2+-derived DBB-Ru ligands (bpy = 2,2′-bipyridine). The Hf12-Ru nMOL was then covalently functionalized with pH-sensitive fluorescein isothiocyanate and pH/oxygen-independent Rhodamine-B isothiocyanate through thiourea linkages to afford Hf12-Ru-F/R as a mitochondria-targeted ratiometric sensor for pH and O2 in live cells. High-resolution confocal microscope imaging with Hf12-Ru-F/R revealed a positive correlation between pH and local O2 concentration in mitochondria. Our work shows the potential of nMOL-based ratiometric biosensors in sensing and imaging of biologically important analytes in live cells.
ISSN:0002-7863
1520-5126
DOI:10.1021/jacs.9b11024