Morphological and functional changes in neonatally X-irradiated thyroid gland in rats

• Published in: ENDOCRINE JOURNAL 2020, Vol.67(2), pp.231-240
• Main Authors: Fujimoto, Nariaki, Matsuu-Matsuyama, Mutsumi, Nakashima, Masahiro
• Format: Article
• Language: English
• Subjects: Age Factors; Animals; Animals, Newborn; Blotting, Western; Cervix; Children; Dose-Response Relationship, Radiation; Epithelium; Follicles; Iodide peroxidase; Iodide Peroxidase - genetics; Iodide Peroxidase - metabolism; Iodide Peroxidase - radiation effects; Male; Morphology; mRNA; Neck; Neonatal irradiation; Neonates; Radiation Dosage; Rats; Rats, Wistar; Reverse Transcriptase Polymerase Chain Reaction; Risk factors; RNA, Messenger - metabolism; RNA, Messenger - radiation effects; Rodents; Serum TSH; Thyroglobulin; Thyroglobulin - genetics; Thyroglobulin - metabolism; Thyroglobulin - radiation effects; Thyroglobulin expression; Thyroid cancer; Thyroid follicles; Thyroid gland; Thyroid Gland - metabolism; Thyroid Gland - pathology; Thyroid Gland - radiation effects; Thyroid-stimulating hormone; Thyrotropin - metabolism; Thyrotropin - radiation effects; Thyroxine; Thyroxine - metabolism; Thyroxine - radiation effects; Triiodothyronine; Triiodothyronine - metabolism; Triiodothyronine - radiation effects; Wistar rats; X-Rays
• ISSN: 0918-8959; 1348-4540
• DOI: 10.1507/endocrj.EJ19-0245

Exposure to ionized radiation in childhood has been recognized as a risk factor for the development of thyroid cancer and possibly for other thyroid disorders. However, the effects of neonatal radiation exposure on thyroid morphology and functions have never been explored despite its potential importance. One-week-old male Wistar rats were subjected to cervical X-irradiation at 6 and 12 Gy. Animals were examined at the ages of 2, 8 and 18 weeks old. For comparison, 8-week-old rats were cervically X-irradiated at the same doses. Thyroid histology was examined by computer-assisted microscopy to measure areas of colloid and epithelium of thyroid follicles as well as epithelial heights. In rats that received cervical X-irradiation at 1 week old, the colloid size of thyroid follicles decreased at the age of 8 weeks old in a radiation-dose dependent manner. This morphological change was persistently found at 18 weeks old. There were no significant differences in serum total T3 or T4 levels among the groups. Serum TSH levels increased significantly in 8-week-old rats neonatally X-irradiated. Thyroglobulin (Tg) mRNA and protein expressions were significantly decreased in the neonatally-irradiated group while thyroid peroxidase mRNA express increased at 18 weeks old. None of these changes were observed in the rats X-irradiated at 8 weeks old. In conclusion, our results clearly demonstrated that neonatal rat thyroid was sensitive to ionized radiation, developing specific morphological changes characterized by smaller thyroid follicles along with changes in serum TSH levels and Tg expressions in the thyroid tissue.