Association between lncRNA and GCKR gene in type 2 diabetes mellitus

•Between T2DM and control group, we identified 68 lncRNAs and 74 mRNAs differentially expressed from the expression profile.•Compared with the control group, the expression levels of the GCKR gene and lncRNA ENST00000588707.1 and TCONS_00004187 in the T2DM group were significantly lower.•The correla...

Full description

Saved in:
Bibliographic Details
Published in:Clinica chimica acta 2020-02, Vol.501, p.66-71
Main Authors: Ma, Qi, Wang, Li, Yang, Ye, Su, Yinxia, Wang, Tingting, Hou, Qinqin, Cai, Ren, Wang, Zhiqiang, Yao, Hua
Format: Article
Language:English
Subjects:
Biomarker
Glucokinase regulatory protein gene
Long non-coding RNA
Type 2 diabetes mellitus
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•Between T2DM and control group, we identified 68 lncRNAs and 74 mRNAs differentially expressed from the expression profile.•Compared with the control group, the expression levels of the GCKR gene and lncRNA ENST00000588707.1 and TCONS_00004187 in the T2DM group were significantly lower.•The correlation analysis revealed that ENST00000588707.1 and TCONS_00004187 were correlated with GCKR gene expression and glycolipid metabolism.•ROC analysis showed that lncRNA ENST0000588707.1 and TCONS_00004187 could serve as potential biomarkers for T2DM. To screen long non-coding RNA (lncRNA) related to glucokinase regulatory protein gene (GCKR), its differential expression was analyzed in patients with Type 2 diabetes mellitus (T2DM) and control samples. The correlation of lncRNA with GCKR was verified and its potential value as a molecular marker of T2DM was assessed. Lymphocyte RNA was extracted from five patients with T2DM and five patients with non-T2DM. The expression profiles of circulating lncRNAs and mRNAs were obtained by microarray. Bioinformatics analysis was used to screen lncRNAs associated with the GCKR gene in 127 patients with T2DM and 130 patients with non-T2DM were selected. The expression levels of the GCKR gene and lncRNA (ENST00000588707.1 and TCONS_00004187) in the T2DM group and control group were verified by real-time PCR. Additionally, a correlation analysis was conducted. The value of circulating ENST00000588707.1 and TCONS_00004187 as biomarkers for the diagnosis of T2DM was performed by receiver operating characteristic curve analysis. We identified 68 lncRNAs and 74 mRNAs differentially expressed from the expression profile. Compared with the control group, the expression levels of the GCKR gene and lncRNA ENST00000588707.1 and TCONS_00004187 in the T2DM group were significantly lower (P 
ISSN:0009-8981
1873-3492
DOI:10.1016/j.cca.2019.10.004