Effect of PR-957 on the formation of A1 reactive astrocytes

• Published in: Zhongguo dang dai er ke za zhi 2019-11, Vol.21 (11), p.1110-1115
• Main Authors: Dai, Shu-Xin, Wang, Yu, Lin, Li-Fang, Yuan, Tian-Ming
• Format: Article
• Language: Chinese
• ISSN: 1008-8830
• DOI: 10.7499/j.issn.1008-8830.2019.11.011

OBJECTIVETo study the effect of PR-957 on the formation of A1 reactive astrocytes. METHODSThe cerebral cortices of 1-day-old female rats were obtained and cultured for primary astrocytes. These cells were divided into 3 groups: control, lipopolysaccharide (LPS), and LPS+PR-957. The LPS group was treated with LPS (at a concentration of 5 μmol/L) for 48 hours; the LPS+PR-957 group was treated with PR-957 (at a final concentration of 200 nmol/L) for 1 hour and then LPS for 48 hours. Enzyme-linked immunosorbent assay was used to determine the expression of complement 3 (C3, a marker for A1 reactive astrocytes) and tumor necrosis factor alpha (TNF-α). Quantitative real-time PCR was used to determine the relative mRNA expression of glypican-6 (GPC6), SPARC-like 1 (SPARCL1), and lipocalin-2 (LCN2). All the above experiments were repeated three times independently. RESULTSC3 expression was almost not observed in the control group, but was observed in both the LPS group and the LPS+PR-957 group, with significantly low