Microvirga sp. symbiovar mediterranense nodulates Lupinus cosentinii grown wild in Morocco

• Published in: Journal of applied microbiology 2020-04, Vol.128 (4), p.1109-1118
• Main Authors: Missbah El Idrissi, M., Lamin, H., ElFaik, S., Tortosa, G., Peix, A., Bedmar, E.J., Abdelmoumen, H.
• Format: Article
• Language: English
• Subjects: agriculture; Cork; diversity; DNA, Bacterial - genetics; DnaK protein; Endosymbionts; Fingerprinting; Genes; Genes, Essential - genetics; Geographical distribution; Lupinus; Lupinus - classification; Lupinus - microbiology; Methylobacteriaceae - classification; Methylobacteriaceae - genetics; Methylobacteriaceae - isolation & purification; Methylobacteriaceae - physiology; microbial phylogenetics; Microvirga; Morocco; Nodulation; Nodules; Nucleotides; Phylogenetics; Phylogeny; RecA protein; RNA, Ribosomal, 16S - genetics; Root nodules; Root Nodules, Plant - microbiology; RpoB protein; rRNA 16S; Sequence Analysis, DNA; soil; Soil chemistry; Soil pH; Strains (organisms); Symbiosis
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/jam.14526

Aim To analyse the diversity of nodule‐forming bacteria isolated from Lupinus cosentinii naturally grown in the Maamora cork oak forest (Rabat, Morocco). Methods and Results Of the 31 bacterial strains, four were selected based on their REP‐PCR fingerprinting that were studied by sequencing and phylogenetic analysis of their 16S rRNA, gyrB, dnaK, recA and rpoB housekeeping genes as well as the nodC symbiotic gene. The nearly complete 16S rRNA gene sequence of the four representative strains showed that they are related to Tunisian strains of genus Microvirga isolated from L. micranthus with nucleotide identity values ranging from 98·67 to 97·13%. The single and concatenated sequences of the 16S rRNA, gyrB, dnaK, recA and rpoB housekeeping genes indicated that the L. cosentinii‐isolated strains had 99·2–99·9% similarities with the Tunisian L. micranthus microsymbionts. The nodC gene phylogeny revealed that the Moroccan strains clustered in the newly described mediterranense symbiovar, and nodulation tests showed that they nodulated not only L. cosentinii but also L. angustifolius, L. luteus and L. albus. Conclusions To the best of our knowledge, this is the first report concerning the isolation, molecular identification and phylogenetic diversity of L. cosentinii nodule‐forming endosymbionts and of their description as members of the Microvirga genus. Significance and Impact of the Study In this work, we show that Microvirga sp. can be isolated from root nodules of wild‐grown L. cosentinii in Northeast Africa, that selected strains also nodulate L. angustifolius, L. luteus and L. albus, and that they belong to symbiovar mediterranense. In addition, our data support that the ability of Microvirga to nodulate lupines could be related to the soil pH, its geographical distribution being more widespread than expected.