Virus susceptibility of a new cell line derived from the fin of black carp Mylopharyngodon piceus

• Published in: Journal of fish biology 2020-02, Vol.96 (2), p.418-426
• Main Authors: Wang, Yizhou, Xue, Ting, Wang, Qian, Xia, Bilin, Pan, Qihua, Chen, Tiansheng
• Format: Article
• Language: English
• Subjects: Animal Fins - cytology; Animal Fins - metabolism; Animal Fins - virology; Animals; Baculovirus; Biotechnology; black carp; Carp; cell line; Cell Line - metabolism; Cell Line - virology; Chromosome number; Chromosomes; Cyprinidae - metabolism; Cyprinidae - virology; Diploids; Electroporation; fin; Fish Diseases - virology; Fish Proteins - genetics; Fish Proteins - metabolism; Fishes; Fluorescence; Freshwater fishes; Gene Expression; Genetic analysis; Genetic Markers - genetics; Genetic Markers - physiology; Genetic Predisposition to Disease; Host Microbial Interactions; Mylopharyngodon piceus; Nanog Homeobox Protein - genetics; Nanog Homeobox Protein - metabolism; Nucleic acids; Nucleotide sequence; Oct-4 protein; Pathogens; Pluripotency; Pluripotent Stem Cells - metabolism; Pluripotent Stem Cells - virology; Primary Cell Culture - methods; Rhabdoviridae - growth & development; Rhabdoviridae Infections - virology; Ribonucleic acid; RNA; rRNA; Storage; SVCV; Transfection; Transfection - methods; Viability; Viremia; Viruses
• ISSN: 0022-1112; 1095-8649
• DOI: 10.1111/jfb.14215

A continuous cell line MPF derived from the fin of black carp Mylopharyngodon piceus was established and characterised in this study. Mylopharyngodon piceus fin (MPF) cells were subcultured for more than 80 passages with high viability recovery after long‐term storage. The karyotyping analysis revealed that MPF had a modal diploid chromosome number (2n = 48) and identical ribosomal RNA sequence with black carp. In addition, the expression of pluripotency‐associated markers including nanog, oct4 and vasa, were detected in MPF. The transient transfection efficiency of MPF reached 23% with a fluorescent reporter by modified electroporation and stable expression of red fluorescent MPF was established by the baculovirus system, indicating that MPF is an ideal platform for studying gene functions in vitro. Lastly, cytopathic effects were also observed and RNA transcripts of a viral gene increased after infection by spring viremia of carp virus (SVCV), suggesting that MPF could be an alternative tool for investigating pathogen‐host interactions in black carp. In conclusion, a fin cell line that is susceptible to SVCV was established as a potential adult stem‐cell line, providing a suitable tool for future genetic analyses and pathogen–host studies in black carp.