DNA methylation profiling to explore colorectal tumor differences according to menopausal hormone therapy use in women

Use of menopausal hormone therapy (MHT) has been associated with a reduced risk for colorectal cancer, but mechanisms underlying this relationship are not well understood. In the colon, MHT appears to act through estrogen receptor β (ERβ) which may influence DNA methylation by binding to DNA. Using...

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Bibliographic Details
Published in:Epigenomics 2019-12, Vol.11 (16), p.1765-1778
Main Authors: Neumeyer, Sonja, Popanda, Odilia, Butterbach, Katja, Edelmann, Dominic, Bläker, Hendrik, Toth, Csaba, Roth, Wilfried, Herpel, Esther, Jäkel, Cornelia, Schmezer, Peter, Benner, Axel, Burwinkel, Barbara, Hoffmeister, Michael, Brenner, Hermann, Chang-Claude, Jenny
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
ALDH1A3
Cancer
Colon
Colorectal cancer
Colorectal carcinoma
Colorectal Neoplasms - genetics
Colorectal Neoplasms - metabolism
CpG Islands
Datasets
Deoxyribonucleic acid
differential methylation
DNA
DNA fingerprinting
DNA Methylation
Endocrine therapy
ERβ
estrogen
Estrogen Receptor beta - metabolism
Estrogen Replacement Therapy
Estrogens
Female
genome-wide
Genomes
Genomics
hormone replacement therapy
Humans
LAPTM4A
Measurement methods
Medical research
Menopause
Middle Aged
Risk management
ST6GALNAC2
Studies
Therapy
Tumors
Womens health
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Summary:Use of menopausal hormone therapy (MHT) has been associated with a reduced risk for colorectal cancer, but mechanisms underlying this relationship are not well understood. In the colon, MHT appears to act through estrogen receptor β (ERβ) which may influence DNA methylation by binding to DNA. Using genome-wide methylation profiling data, we aimed to identify genes that may be differentially methylated according to MHT use. DNA methylation was measured using Illumina HumanMethylation450k arrays in two independent tumor sample sets of colorectal cancer patients. Differential methylation was determined using R/limma. In the discovery analysis, two CpG sites showed differential DNA methylation according to MHT use, both were not replicated. In stratified analyses, 342 CpG sites were associated with current MHT use only in ERβ-positive tumors. The suggestive findings of differential methylation according to current MHT use in ERβ-positive tumors warrant further investigation.
ISSN:1750-1911
1750-192X
DOI:10.2217/epi-2019-0051