Biosynthesis and characterization of zinc oxide nanoparticles from Artemisia annua and investigate their effect on proliferation, osteogenic differentiation and mineralization in human osteoblast-like MG-63 Cells

• Published in: Journal of photochemistry and photobiology. B, Biology Biology, 2020-01, Vol.202, p.111652-111652, Article 111652
• Main Authors: Wang, Dongyan, Cui, Lihuang, Chang, Xin, Guan, Dehong
• Format: Article
• Language: English
• Subjects: A. annua ZnO-NPs; Anti-Infective Agents - chemical synthesis; Anti-Infective Agents - chemistry; Anti-Infective Agents - pharmacology; Antiinfectives and antibacterials; Artemisia annua; Artemisia annua - chemistry; Artemisia annua - metabolism; Assaying; Bioactive compounds; Biocompatibility; Biomedical materials; Biosynthesis; Bone growth; Bone remodeling; Calcium - metabolism; Candida - drug effects; Cell differentiation; Cell Differentiation - drug effects; Cell Line; Cell proliferation; Cell Proliferation - drug effects; Cytotoxicity; Differentiation (biology); Fourier transforms; Gram-Negative Bacteria - drug effects; Gram-Positive Bacteria - drug effects; Green Chemistry Technology; Humans; Infrared spectroscopy; Medical treatment; Metal Nanoparticles - chemistry; Metal Nanoparticles - toxicity; MG-63 cells; Mineralization; Multiplication; Nanoparticles; Osteoblastogenesis; Osteoblasts - cytology; Osteoblasts - metabolism; Osteoclastogenesis; Osteogenesis; Osteogenesis - drug effects; Plant Bark - chemistry; Plant Bark - metabolism; Plant Extracts - chemistry; Spectrum analysis; Test procedures; Transmission electron microscopy; Viability; X-ray diffraction; Zinc; Zinc oxide; Zinc Oxide - chemistry; Zinc oxide nanoparticles (ZnO NPs); Zinc oxides
• ISSN: 1011-1344; 1873-2682
• DOI: 10.1016/j.jphotobiol.2019.111652

The Biocompatibility and stability of nanoparticles using plants have been widely investigated due to its applications in the biomedical industry. Currently, there is a growing interest in nanoparticles in bone remodelling. Artemisia annua is an herbal plant commonly used in the treatment of various ailments. This study investigated the zinc oxide nanoparticles (ZnO NPs) using the green synthesis technique from A. annua and the effects of A. annua ZnO-NPs on osteoblast differentiation and inhibition of osteoclast formation. The formulated ZnO-NPs from A. annua were characterized by using various spectroscopic and microscopic methods Fourier transform-infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), X-ray diffraction (XRD), and UV–Visible spectroscopy. The disc diffusion method was adopted to test the antimicrobial efficacy of ZnO-NPs. The viability of MG-63 cells were assayed by MTT test and Osteogenic-related assays like Real-time PCR and Mineralization assay were adopted to determine the effects of A. annua ZnO-NPs on the multiplication and differentiation of human osteoblast-like MG-63 cells. The characterization of A. annua ZnO-NPs revealed the crystalline nature with high zinc content and the presence of bioactive compounds from A. annua extract. The synthesized A. annua ZnO-NPs indicate significant antimicrobial potential. Besides, A. annua ZnO-NPs enhanced the proliferation, differentiation, and mineralization without causing significant cytotoxic impact on MG-63 cells. These effects indicate that A. annua ZnO-NPs can both stimulate bone formation via the differentiation of MG-63 cells. Hence, it was concluded that A. annua ZnO-NPs can be a promising agent for the treatment of bone deformities and bone-related diseases, however further research also required to explore the clear mechanism of A. annua ZnO-NPs in the formation and differentiation of MG-63 cells. •Artemisia annua is an herbal plant commonly used in the treatment of various ailments.•Osteoporosis is a musculoskeletal ailment, marked by decreased bone mass or low bone mineral density.•These effects indicate that A. annua ZnO-NPs can both stimulate bone formation via the differentiation of MG-63 cells.