The effect of nitrite and sulfide on the antioxidant capacity and microbial composition of the intestines of red swamp crayfish, Procambarus clarkii

• Published in: Fish & shellfish immunology 2020-01, Vol.96, p.290-296
• Main Authors: Guo, Kun, Ruan, Guoliang, Fan, Wenhao, Fang, Liu, Wang, Qian, Luo, Mingzhong, Yi, Tilin
• Format: Article
• Language: English
• Subjects: Animals; Antioxidant gene expression; Antioxidants - metabolism; Astacoidea - drug effects; Astacoidea - microbiology; Astacoidea - physiology; Gastrointestinal Microbiome - drug effects; Gastrointestinal Microbiome - physiology; Gene Expression - drug effects; Immunity, Innate - drug effects; Intestinal microbiota; Nitrite stress; Nitrites - toxicity; Oxidative stress; Oxidative Stress - drug effects; Procambarus clarkii; Random Allocation; Sulfide stress; Sulfides - toxicity
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2019.11.052

Nitrite and sulfide are harmful pollutants in water ecosystems that negatively influence the survival and growth of crayfish. It is currently known that the intestine of crustaceans acts as a significant immune organ, serving as the front line of defense against diseases. In this study, we investigated how the oxidative damage parameters, antioxidant status and microbial composition of the intestine of Procambarus clarkii were influenced under acute nitrite (60 mg/L) and sulfide (18 mg/L) stress for 72 h. Compared with the control, after exposure to nitrite and sulfide stress, the production of reactive oxygen species, and the lipid peroxide and malondialdehyde contents increased in the intestines and were significantly higher after 72 h of exposure. The superoxide dismutase, catalase and glutathione peroxidase activities increased to maximum levels at 6, 24 and 12 h, respectively. These activities then decreased gradually and were significantly lower than those of the control after 48 or 72 h of exposure. In the crayfish exposed to stress, the expression of antioxidant genes including heat shock protein 70, ferritin and metallothionein increased to their maximum values at 12, 48 and 12 h, respectively. The expression levels then decreased gradually, and after 72 h, were lower than, or lacked significant differences with, the expression levels in the control. Additionally, nitrite and sulfide exposure restructured the intestinal microbial community of P. clarkii. This led to decreases in the abundance of some genera such as Citrobacter. However, the abundance of other genera, such as Shewanella and Acinetobacter, increased. Therefore, the health of P. clarkii was seriously impaired when exposed to nitrite and sulfide stress. •Nitrite and sulfide stress increased oxidative stress in crayfish intestines.•Antioxidant enzyme activity was reduced after 72 h of nitrite and sulfide stress.•Antioxidant gene transcription was reduced after nitrite and sulfide accumulation.•The diversity and richness of the intestinal microbiome decreased after stress.•There were higher proportions of pathogenic bacterial genera following stress.