Crocin, an active constituent of Crocus sativus ameliorates cerulein induced pancreatic inflammation and oxidative stress

Acute pancreatitis (AP) is a disorder of the pancreas marked by profound inflammation and oxidative stress. Phytoconstituents presents an important toolbox of preventive strategies to combat inflammatory disorders. To this end, we selected the active constituent of Crocus sativus, crocin for evaluat...

Full description

Saved in:
Bibliographic Details
Published in:Phytotherapy research 2020-04, Vol.34 (4), p.825-835
Main Authors: Godugu, Chandraiah, Pasari, Lakshmi P., Khurana, Amit, Anchi, Pratibha, Saifi, Mohd A., Bansod, Sapana P., Annaldas, Shivaraju
Format: Article
Language:English
Subjects:
acute pancreatitis
Anti-inflammatory agents
cerulein
Constituents
crocin
Crocus sativus
Cytokines
Edema
Histology
Immunohistochemistry
Inflammatory diseases
Lipase
NF-κB protein
Nitrotyrosine
Nuclear transport
Oxidative stress
Pancreas
Pancreatitis
Saffron
Translocation
Tumor necrosis factor
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Acute pancreatitis (AP) is a disorder of the pancreas marked by profound inflammation and oxidative stress. Phytoconstituents presents an important toolbox of preventive strategies to combat inflammatory disorders. To this end, we selected the active constituent of Crocus sativus, crocin for evaluation against cerulein‐induced AP, owing to its promising antiinflammatory activity in acute as well as chronic inflammatory conditions. The animals were randomly divided into five groups comprising of normal control, cerulein control, crocin low dose (30 mg/kg), crocin high dose (100 mg/kg), and crocin control (100 mg/kg). Various biochemical parameters and the levels of inflammatory cytokines and p65‐NFκB were measured. The mechanism was investigated by histology and immunohistochemistry. We found that crocin significantly reduced the pancreatic edema, amylase, and lipase levels. It abrogated the oxidative stress incurred by cerulein challenge. We found that crocin modulated the pancreatic inflammatory cytokine levels. Crocin perturbed the nuclear translocation of p65‐NFκB. Crocin reverted the pancreatic histology associated with AP. Furthermore, it upregulated the expression of Nrf‐2 and downregulated the expression of IL‐6, TNF‐α, nitrotyrosine, and NFκB. Cumulatively, these results indicate that crocin has promising potential to prevent cerulein induced AP and regular intake of saffron can prove beneficial for the pancreatic health.
ISSN:0951-418X
1099-1573
DOI:10.1002/ptr.6564