Restriction enzymes and their use in molecular biology: An overview

Restriction enzymes have been identified in the early 1950s of the past century and have quickly become key players in the molecular biology of DNA. Forty years ago, the scientists whose pioneering work had explored the activity and sequence specificity of these enzymes, contributing to the definiti...

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Bibliographic Details
Published in:Journal of biosciences 2019-06, Vol.44 (2), p.1-8, Article 38
Main Authors: Di Felice, Francesca, Micheli, Gioacchino, Camilloni, Giorgio
Format: Article
Language:English
Subjects:
Biology
Biomedical and Life Sciences
Biomedicine
Cell Biology
Deoxyribonucleic acid
DNA
Enzymes
Life Sciences
Mapping
Microbiology
Molecular biology
Nucleotide sequence
Plant Sciences
Proteins
Review
Specificity
Zoology
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Summary:Restriction enzymes have been identified in the early 1950s of the past century and have quickly become key players in the molecular biology of DNA. Forty years ago, the scientists whose pioneering work had explored the activity and sequence specificity of these enzymes, contributing to the definition of their enormous potential as tools for DNA characterization, mapping and manipulation, were awarded the Nobel Prize. In this short review, we celebrate the history of these enzymes in the light of their many different uses, as these proteins have accompanied the history of DNA for over 50 years representing active witnesses of major steps in the field.
ISSN:0250-5991
0973-7138
DOI:10.1007/s12038-019-9856-8