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Identification of Mycobacterium abscessus species and subspecies using the Cas12a/sgRNA-based nucleic acid detection platform
The rapidly growing mycobacterium Mycobacterium abscessus is a clinically important organism causing pulmonary and skin diseases. The M. abscessus complex is comprised of three subspecies: M. abscessus subsp. abscessus , M. abscessus subsp. massiliense , and M. abscessus subsp. bolletii . Here, we a...
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| Published in: | European journal of clinical microbiology & infectious diseases 2020-03, Vol.39 (3), p.551-558 |
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| Main Authors: | , , , , , , , , , , , , |
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| container_end_page | 558 |
| container_issue | 3 |
| container_start_page | 551 |
| container_title | European journal of clinical microbiology & infectious diseases |
| container_volume | 39 |
| creator | Xiao, Guohui Zhang, Su Liang, Zhihang Li, Guanqiang Fang, Mutong Liu, Yaya Zhang, Juanjuan Ou, Min He, Xing Zhang, Tianyu Zeng, Changchun Liu, Lei Zhang, Guoliang |
| description | The rapidly growing mycobacterium
Mycobacterium abscessus
is a clinically important organism causing pulmonary and skin diseases. The
M. abscessus
complex is comprised of three subspecies:
M. abscessus
subsp.
abscessus
,
M. abscessus
subsp.
massiliense
, and
M. abscessus
subsp.
bolletii
. Here, we aimed to develop a Cas12a/sgRNA-based nucleic acid detection platform to identify
M
.
abscessus
species and subspecies. By designing specific sgRNA probes targeting
rpoB
and
erm
(41), we demonstrated that
M. abscessus
could be differentiated from other major mycobacterial species and identified at the subspecies level. Using this platform, a total of 38 clinical
M. abscessus
isolates were identified, 18 as
M. abscessus
subsp.
abscessus
and 20 as
M. abscessus
subsp.
massiliense
. We concluded that the Cas12a/sgRNA-based nucleic acid detection platform provides an easy-to-use, quick, and cost-effective approach for identification of
M. abscessus
species and subspecies. |
| doi_str_mv | 10.1007/s10096-019-03757-y |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2319496428</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2362197757</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-a69a15efb28481efd970bdfc6211008ce0127181a1b8bf3d7424fee99f7e10e63</originalsourceid><addsrcrecordid>eNp9kTtvVDEQhS0EIkvgD1AgSzQ0Jn7t9XUZrQJECiAhqC3bd7w4uo_Fc11swX_HZBOQKGhsWf7OnJk5hLwU_K3g3FxgO23HuLCMK7M17PiIbIRWW6aVUY_JhlulmTVSnZFniLe8iXpjnpIzJYzpeqM35Of1APOaU45-zctMl0Q_HuMSfFyh5DpRHzACYkWKB4gZkPp5oFjDw7Ninvd0_Q5051FIf4H7L58uWfAIA51rHCFH6mMe6AArxDuXw-jXtJTpOXmS_Ijw4v4-J9_eXX3dfWA3n99f7y5vWGxzrcx31ostpCB73QtIgzU8DCl2UrQV9BG4kEb0wovQh6QGo6VOANYmA4JDp87Jm1PdQ1l-VMDVTbmNNY5-hqWik0pYbTst-4a-_ge9XWqZW3eNaobWtE03Sp6oWBbEAskdSp58OTrB3e9w3Ckc18Jxd-G4YxO9ui9dwwTDH8lDGg1QJwDb17yH8tf7P2V_AagFnCI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2362197757</pqid></control><display><type>article</type><title>Identification of Mycobacterium abscessus species and subspecies using the Cas12a/sgRNA-based nucleic acid detection platform</title><source>Springer Link</source><creator>Xiao, Guohui ; Zhang, Su ; Liang, Zhihang ; Li, Guanqiang ; Fang, Mutong ; Liu, Yaya ; Zhang, Juanjuan ; Ou, Min ; He, Xing ; Zhang, Tianyu ; Zeng, Changchun ; Liu, Lei ; Zhang, Guoliang</creator><creatorcontrib>Xiao, Guohui ; Zhang, Su ; Liang, Zhihang ; Li, Guanqiang ; Fang, Mutong ; Liu, Yaya ; Zhang, Juanjuan ; Ou, Min ; He, Xing ; Zhang, Tianyu ; Zeng, Changchun ; Liu, Lei ; Zhang, Guoliang</creatorcontrib><description>The rapidly growing mycobacterium
Mycobacterium abscessus
is a clinically important organism causing pulmonary and skin diseases. The
M. abscessus
complex is comprised of three subspecies:
M. abscessus
subsp.
abscessus
,
M. abscessus
subsp.
massiliense
, and
M. abscessus
subsp.
bolletii
. Here, we aimed to develop a Cas12a/sgRNA-based nucleic acid detection platform to identify
M
.
abscessus
species and subspecies. By designing specific sgRNA probes targeting
rpoB
and
erm
(41), we demonstrated that
M. abscessus
could be differentiated from other major mycobacterial species and identified at the subspecies level. Using this platform, a total of 38 clinical
M. abscessus
isolates were identified, 18 as
M. abscessus
subsp.
abscessus
and 20 as
M. abscessus
subsp.
massiliense
. We concluded that the Cas12a/sgRNA-based nucleic acid detection platform provides an easy-to-use, quick, and cost-effective approach for identification of
M. abscessus
species and subspecies.</description><identifier>ISSN: 0934-9723</identifier><identifier>EISSN: 1435-4373</identifier><identifier>DOI: 10.1007/s10096-019-03757-y</identifier><identifier>PMID: 31776874</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Bacterial Proteins - genetics ; Biomedical and Life Sciences ; Biomedicine ; CRISPR-Associated Proteins - genetics ; CRISPR-Cas Systems ; DNA, Bacterial ; Endodeoxyribonucleases - genetics ; Genes, Bacterial ; Humans ; Internal Medicine ; Lung diseases ; Medical Microbiology ; Molecular Typing - methods ; Mycobacterium abscessus ; Mycobacterium abscessus - classification ; Mycobacterium abscessus - genetics ; Mycobacterium Infections, Nontuberculous - diagnosis ; Mycobacterium Infections, Nontuberculous - microbiology ; Nucleic acids ; Original Article ; Phylogeny ; Polymerase Chain Reaction ; RNA, Guide, CRISPR-Cas Systems ; RpoB protein ; Sequence Analysis, DNA ; Skin diseases ; Species ; Workflow</subject><ispartof>European journal of clinical microbiology & infectious diseases, 2020-03, Vol.39 (3), p.551-558</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2019</rights><rights>European Journal of Clinical Microbiology and Infectious Diseases is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-a69a15efb28481efd970bdfc6211008ce0127181a1b8bf3d7424fee99f7e10e63</citedby><cites>FETCH-LOGICAL-c375t-a69a15efb28481efd970bdfc6211008ce0127181a1b8bf3d7424fee99f7e10e63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31776874$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xiao, Guohui</creatorcontrib><creatorcontrib>Zhang, Su</creatorcontrib><creatorcontrib>Liang, Zhihang</creatorcontrib><creatorcontrib>Li, Guanqiang</creatorcontrib><creatorcontrib>Fang, Mutong</creatorcontrib><creatorcontrib>Liu, Yaya</creatorcontrib><creatorcontrib>Zhang, Juanjuan</creatorcontrib><creatorcontrib>Ou, Min</creatorcontrib><creatorcontrib>He, Xing</creatorcontrib><creatorcontrib>Zhang, Tianyu</creatorcontrib><creatorcontrib>Zeng, Changchun</creatorcontrib><creatorcontrib>Liu, Lei</creatorcontrib><creatorcontrib>Zhang, Guoliang</creatorcontrib><title>Identification of Mycobacterium abscessus species and subspecies using the Cas12a/sgRNA-based nucleic acid detection platform</title><title>European journal of clinical microbiology & infectious diseases</title><addtitle>Eur J Clin Microbiol Infect Dis</addtitle><addtitle>Eur J Clin Microbiol Infect Dis</addtitle><description>The rapidly growing mycobacterium
Mycobacterium abscessus
is a clinically important organism causing pulmonary and skin diseases. The
M. abscessus
complex is comprised of three subspecies:
M. abscessus
subsp.
abscessus
,
M. abscessus
subsp.
massiliense
, and
M. abscessus
subsp.
bolletii
. Here, we aimed to develop a Cas12a/sgRNA-based nucleic acid detection platform to identify
M
.
abscessus
species and subspecies. By designing specific sgRNA probes targeting
rpoB
and
erm
(41), we demonstrated that
M. abscessus
could be differentiated from other major mycobacterial species and identified at the subspecies level. Using this platform, a total of 38 clinical
M. abscessus
isolates were identified, 18 as
M. abscessus
subsp.
abscessus
and 20 as
M. abscessus
subsp.
massiliense
. We concluded that the Cas12a/sgRNA-based nucleic acid detection platform provides an easy-to-use, quick, and cost-effective approach for identification of
M. abscessus
species and subspecies.</description><subject>Bacterial Proteins - genetics</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>CRISPR-Associated Proteins - genetics</subject><subject>CRISPR-Cas Systems</subject><subject>DNA, Bacterial</subject><subject>Endodeoxyribonucleases - genetics</subject><subject>Genes, Bacterial</subject><subject>Humans</subject><subject>Internal Medicine</subject><subject>Lung diseases</subject><subject>Medical Microbiology</subject><subject>Molecular Typing - methods</subject><subject>Mycobacterium abscessus</subject><subject>Mycobacterium abscessus - classification</subject><subject>Mycobacterium abscessus - genetics</subject><subject>Mycobacterium Infections, Nontuberculous - diagnosis</subject><subject>Mycobacterium Infections, Nontuberculous - microbiology</subject><subject>Nucleic acids</subject><subject>Original Article</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction</subject><subject>RNA, Guide, CRISPR-Cas Systems</subject><subject>RpoB protein</subject><subject>Sequence Analysis, DNA</subject><subject>Skin diseases</subject><subject>Species</subject><subject>Workflow</subject><issn>0934-9723</issn><issn>1435-4373</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kTtvVDEQhS0EIkvgD1AgSzQ0Jn7t9XUZrQJECiAhqC3bd7w4uo_Fc11swX_HZBOQKGhsWf7OnJk5hLwU_K3g3FxgO23HuLCMK7M17PiIbIRWW6aVUY_JhlulmTVSnZFniLe8iXpjnpIzJYzpeqM35Of1APOaU45-zctMl0Q_HuMSfFyh5DpRHzACYkWKB4gZkPp5oFjDw7Ninvd0_Q5051FIf4H7L58uWfAIA51rHCFH6mMe6AArxDuXw-jXtJTpOXmS_Ijw4v4-J9_eXX3dfWA3n99f7y5vWGxzrcx31ostpCB73QtIgzU8DCl2UrQV9BG4kEb0wovQh6QGo6VOANYmA4JDp87Jm1PdQ1l-VMDVTbmNNY5-hqWik0pYbTst-4a-_ge9XWqZW3eNaobWtE03Sp6oWBbEAskdSp58OTrB3e9w3Ckc18Jxd-G4YxO9ui9dwwTDH8lDGg1QJwDb17yH8tf7P2V_AagFnCI</recordid><startdate>20200301</startdate><enddate>20200301</enddate><creator>Xiao, Guohui</creator><creator>Zhang, Su</creator><creator>Liang, Zhihang</creator><creator>Li, Guanqiang</creator><creator>Fang, Mutong</creator><creator>Liu, Yaya</creator><creator>Zhang, Juanjuan</creator><creator>Ou, Min</creator><creator>He, Xing</creator><creator>Zhang, Tianyu</creator><creator>Zeng, Changchun</creator><creator>Liu, Lei</creator><creator>Zhang, Guoliang</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>20200301</creationdate><title>Identification of Mycobacterium abscessus species and subspecies using the Cas12a/sgRNA-based nucleic acid detection platform</title><author>Xiao, Guohui ; Zhang, Su ; Liang, Zhihang ; Li, Guanqiang ; Fang, Mutong ; Liu, Yaya ; Zhang, Juanjuan ; Ou, Min ; He, Xing ; Zhang, Tianyu ; Zeng, Changchun ; Liu, Lei ; Zhang, Guoliang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-a69a15efb28481efd970bdfc6211008ce0127181a1b8bf3d7424fee99f7e10e63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Bacterial Proteins - 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Academic</collection><jtitle>European journal of clinical microbiology & infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xiao, Guohui</au><au>Zhang, Su</au><au>Liang, Zhihang</au><au>Li, Guanqiang</au><au>Fang, Mutong</au><au>Liu, Yaya</au><au>Zhang, Juanjuan</au><au>Ou, Min</au><au>He, Xing</au><au>Zhang, Tianyu</au><au>Zeng, Changchun</au><au>Liu, Lei</au><au>Zhang, Guoliang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of Mycobacterium abscessus species and subspecies using the Cas12a/sgRNA-based nucleic acid detection platform</atitle><jtitle>European journal of clinical microbiology & infectious diseases</jtitle><stitle>Eur J Clin Microbiol Infect Dis</stitle><addtitle>Eur J Clin Microbiol Infect Dis</addtitle><date>2020-03-01</date><risdate>2020</risdate><volume>39</volume><issue>3</issue><spage>551</spage><epage>558</epage><pages>551-558</pages><issn>0934-9723</issn><eissn>1435-4373</eissn><abstract>The rapidly growing mycobacterium
Mycobacterium abscessus
is a clinically important organism causing pulmonary and skin diseases. The
M. abscessus
complex is comprised of three subspecies:
M. abscessus
subsp.
abscessus
,
M. abscessus
subsp.
massiliense
, and
M. abscessus
subsp.
bolletii
. Here, we aimed to develop a Cas12a/sgRNA-based nucleic acid detection platform to identify
M
.
abscessus
species and subspecies. By designing specific sgRNA probes targeting
rpoB
and
erm
(41), we demonstrated that
M. abscessus
could be differentiated from other major mycobacterial species and identified at the subspecies level. Using this platform, a total of 38 clinical
M. abscessus
isolates were identified, 18 as
M. abscessus
subsp.
abscessus
and 20 as
M. abscessus
subsp.
massiliense
. We concluded that the Cas12a/sgRNA-based nucleic acid detection platform provides an easy-to-use, quick, and cost-effective approach for identification of
M. abscessus
species and subspecies.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>31776874</pmid><doi>10.1007/s10096-019-03757-y</doi><tpages>8</tpages></addata></record> |
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| identifier | ISSN: 0934-9723 |
| ispartof | European journal of clinical microbiology & infectious diseases, 2020-03, Vol.39 (3), p.551-558 |
| issn | 0934-9723 1435-4373 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2319496428 |
| source | Springer Link |
| subjects | Bacterial Proteins - genetics Biomedical and Life Sciences Biomedicine CRISPR-Associated Proteins - genetics CRISPR-Cas Systems DNA, Bacterial Endodeoxyribonucleases - genetics Genes, Bacterial Humans Internal Medicine Lung diseases Medical Microbiology Molecular Typing - methods Mycobacterium abscessus Mycobacterium abscessus - classification Mycobacterium abscessus - genetics Mycobacterium Infections, Nontuberculous - diagnosis Mycobacterium Infections, Nontuberculous - microbiology Nucleic acids Original Article Phylogeny Polymerase Chain Reaction RNA, Guide, CRISPR-Cas Systems RpoB protein Sequence Analysis, DNA Skin diseases Species Workflow |
| title | Identification of Mycobacterium abscessus species and subspecies using the Cas12a/sgRNA-based nucleic acid detection platform |
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