Characterization of NCC-RbC-51, an RB cell line isolated from a metastatic site

Retinoblastoma (RB) is a childhood eye tumor, caused by the RB1 gene mutation. Since RB is a rapidly proliferating tumor, the patient presents with a Group-D/E tumor at the time of diagnosis. Enucleation is preferred in most unilateral cases to prevent metastasis. Various cell lines have been establ...

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Bibliographic Details
Published in:Histochemistry and cell biology 2020-02, Vol.153 (2), p.101-109
Main Authors: Ravishankar, Hemanth, Mangani, Abubakar Siddiq, Shankar, M. Bhavani, Joshi, Mayur, Devasena, T., Parameswaran, Sowmya, Subramaniam, Krishnakumar
Format: Article
Language:English
Subjects:
Apoptosis
Biochemistry
Biomedical and Life Sciences
Biomedicine
Cancer
Cell Biology
Cell Proliferation
Children
Copy number
Developmental Biology
DNA Copy Number Variations - genetics
DNA, Neoplasm - genetics
Enucleation
Eye diseases
Growth patterns
Humans
Invasiveness
Lymph nodes
Metastases
Metastasis
Mutation
Original Paper
Photomicrography
Point mutation
Retina
Retinal Neoplasms - genetics
Retinal Neoplasms - pathology
Retinal Neoplasms - secondary
Retinoblastoma
Retinoblastoma - genetics
Retinoblastoma - pathology
Retinoblastoma - secondary
Retinoblastoma Binding Proteins - genetics
Retinoblastoma protein
Tumor cell lines
Tumor Cells, Cultured
Tumors
Ubiquitin-Protein Ligases - genetics
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Summary:Retinoblastoma (RB) is a childhood eye tumor, caused by the RB1 gene mutation. Since RB is a rapidly proliferating tumor, the patient presents with a Group-D/E tumor at the time of diagnosis. Enucleation is preferred in most unilateral cases to prevent metastasis. Various cell lines have been established to study the tumor’s growth pattern and target the cancer cells. The commonly used cell lines are WERI-Rb-1 and Y79, both isolated from the primary tumor of RB. Cell lines established from the metastatic site of RB have not been characterized before. In this study, we have characterized NCC-RbC-51, derived from RB tumor to cervical lymph node site and investigated its potential to represent a highly aggressive and metastatic tumor. We compared the proliferative and invasive properties of NCC-RbC-51 with a cell line isolated from the primary site, WERI-Rb-1. NCC-RbC-51 had higher rates of proliferation and apoptosis and had better invasive ability. Copy number variation analysis and the pathways predicted from these show that the pathways altered in NCC-RbC-51 could contribute to its metastatic nature. In all, the results suggest that NCC-RbC-51, a cell line isolated from metastatic site, could be a potential model to study aggressive/invasive RB.
ISSN:0948-6143
1432-119X
DOI:10.1007/s00418-019-01832-1