Knockdown of long noncoding RNA XIST mitigates the apoptosis and inflammatory injury of microglia cells after spinal cord injury through miR-27a/Smurf1 axis

• Published in: Neuroscience letters 2020-01, Vol.715, p.134649-134649, Article 134649
• Main Authors: Zhao, Qin, Lu, Fei, Su, Qinglun, Liu, Zhen, Xia, Xiaomei, Yan, Zhenzhuang, Zhou, Fang, Qin, Rujie
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis - physiology; bcl-2-Associated X Protein - biosynthesis; Caspase 3 - biosynthesis; Cell Survival - physiology; Gene Silencing; Interleukin-6 - biosynthesis; Lipopolysaccharides; LPS; Male; Microglia - physiology; MicroRNAs - biosynthesis; miR-27a; Primary Cell Culture; Proto-Oncogene Proteins c-bcl-2 - biosynthesis; Rats; RNA, Long Noncoding - biosynthesis; RNA, Long Noncoding - genetics; Smurf1; Spinal Cord Injuries - metabolism; Spinal cord injury (SCI); Tumor Necrosis Factor-alpha - biosynthesis; Ubiquitin-Protein Ligases - biosynthesis; XIST
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/j.neulet.2019.134649

•XIST knockdown suppresses cell apoptosis and inflammatory injury after SCI in vitro and in vivo.•Smurf1 mediates the protective role of XIST deletion in SCI via miR-27a.•XIST/miR-27a/Smurf1 pathway may contribute to the onset of SCI. Spinal cord injury (SCI) is a devastating neuropathological condition. Long noncoding RNA X-inactive specific transcript (XIST) is an acknowledged cancer-related gene and participates in the development of SCI. However, role of XIST in SCI remains to be well revealed. Expression of XIST, miRNA-27a-3p (miR-27a) and smad ubiquitination regulatory factor 1 (Smurf1) was detected using RT-qPCR and western blotting. Cell apoptosis and inflammatory injury were assessed by sulforhodamine B (SRB) assay, flow cytometry, western blotting and enzyme-linked immunosorbent assay. The relationship among miR-27a, XIST and Smurf1 was confirmed by dual-luciferase reporter assay, RNA immunoprecipitation and RNA pull-down assay. As a result, we observed higher level of XIST and Smurf1, but lower level of miR-27a in SCI rats and lipopolysaccharide (LPS)-induced primary microglial cells. in vitro, LPS induced SCI microglia cells as described by decreased cell viability and B cell lymphoma 2 (Bcl-2) expression, and increased cell apoptosis rate, Bax and cleaved caspase 3 levels, and tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) secretions. in vivo, a T10 laminectomy caused SCI rats as evidenced by decreased Basso-Beattie-Bresnahan Locomotor Rating Scale (BBB) score and induced expression of Bax, cleaved caspase 3, TNF-α and IL-6. However, silencing of XIST could mitigate the apoptosis and inflammatory injury in LPS-induced microglia and SCI rats. Mechanically, miR-27a interacted with XIST and Smurf1 via target binding. Either miR-27a downregulation or Smurf1 overexpression partially reversed the role of XIST deletion in LPS-treated microglial cells. Collectively, knockdown of XIST could alleviate the apoptosis and inflammatory injury of SCI models in vitro and in vivo through directly modulating miR-27a/Smurf1 axis.