Evaluation of a bovine cathelicidin ELISA for detecting mastitis in the dairy buffalo: Comparison with milk somatic cell count and bacteriological culture

A recently developed bovine cathelicidin (CATH) ELISA was evaluated in the dairy buffalo (Bubalus bubalis) by testing 618 quarter milk samples from a herd with subclinical mastitis cases. Somatic cell count (SCC) and bacteriological culture (BC) were carried out on the same samples for comparison. O...

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Bibliographic Details
Published in:Research in veterinary science 2020-02, Vol.128, p.129-134
Main Authors: Puggioni, Giulia Maria Grazia, Tedde, Vittorio, Uzzau, Sergio, Guccione, Jacopo, Ciaramella, Paolo, Pollera, Claudia, Moroni, Paolo, Bronzo, Valerio, Addis, Maria Filippa
Format: Article
Language:English
Subjects:
Animals
Antimicrobial Cationic Peptides - chemistry
Bacteriological culture
Bubalus bubalis
Buffaloes
Cathelicidin ELISA
Cattle
Cell Count - veterinary
Cell culture
Diagnostic Tests, Routine
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - standards
Enzyme-Linked Immunosorbent Assay - veterinary
Female
Laboratories
Mastitis
Mastitis - diagnosis
Mastitis - veterinary
Microorganisms
Milk
Milk - chemistry
Milk - cytology
Milk - microbiology
Sensitivity
Sensitivity and Specificity
Software
Somatic cell count
Staphylococcal Infections - diagnosis
Staphylococcal Infections - veterinary
Staphylococcus aureus - growth & development
Statistical analysis
Subclinical mastitis
Veterinary medicine
Water buffalo milk
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Summary:A recently developed bovine cathelicidin (CATH) ELISA was evaluated in the dairy buffalo (Bubalus bubalis) by testing 618 quarter milk samples from a herd with subclinical mastitis cases. Somatic cell count (SCC) and bacteriological culture (BC) were carried out on the same samples for comparison. Out of 618 quarters, 258 (41.75%) were positive to CATH, 289 (46.76%) had SCC > 200,000 cells/mL, and 457 (73.95%) were positive to BC. The most prevalent microorganism was Staphylococcus aureus (SAU, 35.76% of all quarters), followed by non-aureus staphylococci (NAS, 22.17% of all quarters). Clinical mastitis quarters were only 7 (1.13%). CATH levels were significantly higher in clinical quarters and in high SCC, BC-positive quarters than in healthy, low SCC, BC-negative quarters. The highest median values were observed for SAU and the lowest for NAS. Differences among microorganism classes were generally more significant for SCC than for CATH. Test characteristics of the CATH ELISA, evaluated by considering as true positives all BC-positive quarters with SCC > 200,000 cells/mL (N = 242), and as true negatives all sterile quarters with SCC 
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2019.11.009