Synergistic Cytotoxicity Of Shikonin‐Silver Nanoparticles As An Opportunity For Lung Cancer

• Published in: Journal of labelled compounds & radiopharmaceuticals 2020-01, Vol.63 (1), p.25-32
• Main Authors: Fayez, Hend, El‐Motaleb, Mohamed Abd, Selim, Adli Abdullah
• Format: Article
• Language: English
• Subjects: A549 Cells; Anticancer properties; Antineoplastic Agents - chemical synthesis; Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacology; Antitumor activity; Apoptosis; Cell culture; Cell proliferation; Cell Proliferation - drug effects; Cell Survival - drug effects; Cell viability; Combinatorial analysis; Cytotoxicity; Deduction; Drug Synergism; Hazard mitigation; Humans; Iodine; Lung cancer; Lung carcinoma; Lung Neoplasms - drug therapy; Lung Neoplasms - pathology; Metal Nanoparticles - chemistry; Nanoparticles; Naphthoquinones - chemistry; Naphthoquinones - pharmacology; Organic chemistry; radiolabeling; Radiolabelling; Shikonin; Silver; Silver - chemistry; Silver - pharmacology; silver nanoparticle; Tissue culture; Toxicity
• ISSN: 0362-4803; 1099-1344; 1099-1344
• DOI: 10.1002/jlcr.3818

The combined action of shikonin and silver nanoparticles (AgNPs) for apoptosis in human cancer cells has not been elucidated. Hence, we investigated the synergistic combinatorial effect of shikonin and AgNPs in human lung cancer cells. Shikonin was used as a reducing and capping agent for AgNPs synthesis as a green method avoiding the hazards of chemical methods. Radiolabeling of shikonin‐AgNPs with radioactive iodine forming [131I]I‐Shikonin‐AgNPs was carried out to enable the intracellular tracking of NPs. The antitumor effect of a combined treatment (shikonin‐AgNPs) was evaluated using tissue culture assay. The 50% inhibitory concentration (IC50) of SHK‐AgNPs on A549 cells after 24 hours determined by an MTT assay is 2.4 ± 0.11 μg/mL. As a deduction, this study revealed that the combination of shikonin and AgNPs treatment significantly inhibited cell viability and proliferation of A549 cells (human lung carcinoma cell line) with a great potential than the monotherapy. A shikonin silver nanoparticles were synthesized, characterized and tested in‐vitro for there synergestic cytotoxic effect against human lung carcinoma cell line and then tracked in‐vivo to study its biodistribution and affinity to lung cell and showed high affinity to lung tissues. so, shikonin Ag‐NPs may be considered as an opportunity to lung cancer.