Molecular characterization and expression patterns of two hormone-sensitive lipase genes in common carp Cyprinus carpio

The hormone-sensitive lipase ( HSL ) gene plays an important role in mammals’ lipid metabolism. Therefore, its function in fish is capturing increasing attention. In this study, two distinct cDNAs, designated HSL 1 and HSL 2, are firstly identified from common carp Cyprinus carpio . The full-length...

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Published in:Fish physiology and biochemistry 2020-02, Vol.46 (1), p.439-450
Main Authors: Zheng, Xianhu, Dang, Hongyang, Lv, Weihua, Sun, Zhipeng, Kuang, Youyi, Cao, Dingchen, Lu, Cuiyun, Sun, Xiaowen
Format: Article
Language:English
Subjects:
Amino acids
Animal Anatomy
Animal Biochemistry
Animal Physiology
Biomedical and Life Sciences
Body condition
Carp
Complementary DNA
Cyprinus carpio
Developmental stages
DNA
Embryogenesis
Embryonic development
Embryonic growth stage
Fat metabolism
Fish
Freshwater & Marine Ecology
Freshwater fishes
Gene expression
Genes
Genomes
Histology
Hormones
Life Sciences
Lipase
Lipid metabolism
Lipids
Metabolism
Morphology
Phylogeny
Polypeptides
Tissue
Zoology
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Summary:The hormone-sensitive lipase ( HSL ) gene plays an important role in mammals’ lipid metabolism. Therefore, its function in fish is capturing increasing attention. In this study, two distinct cDNAs, designated HSL 1 and HSL 2, are firstly identified from common carp Cyprinus carpio . The full-length cDNA of HSL 1 and HSL 2 consists of 3379 bp and 2732 bp, encoding polypeptide of 693 and 847 amino acids, respectively, and shares 60.6% amino acid identity. Phylogenetic analysis suggests that HSL 1 and HSL 2 are derived from paralogous genes, which might have arisen during a teleost-specific genome duplication event. The two HSL mRNAs are differentially expressed, both in terms of distribution among tissues and in terms of abundance during embryogenesis. Moreover, both HSL mRNAs are expressed in various tissues, the highest in abdominal fat. Meanwhile, the two HSL s are detected at all stages of embryonic development, suggesting that they could be functional and involved in embryogenesis. In addition, the results show that the mRNA expression level of HSL 2 in the high group of intramuscular fat content is significantly higher than that in the low group ( P  
ISSN:0920-1742
1573-5168
DOI:10.1007/s10695-019-00738-y