A multifunctional long‐term release system for treatment of hypothyroidism

Hypothyroidism is an autoimmune disease associated with underactive thyroid gland. In this study, a dual effect polymeric system was designed to release Cepharanthine (CEP) to block T cell activation and Selenium (Se) to decrease the anti‐thyroid peroxidase (TPOAb) concentration in order to treat hy...

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Bibliographic Details
Published in:Journal of biomedical materials research. Part A 2020-03, Vol.108 (3), p.760-759
Main Authors: Kutlu Kaya, Ceren, Gümrükçü, Selin, Saraç, Abdulkadir Sezai, Kök, Fatma Neşe
Format: Article
Language:English
Subjects:
Acetic acid
Autoimmune diseases
Block copolymers
Cell activation
Cell viability
Cepharanthine
Chemical synthesis
controlled release system
Ethylene vinyl acetates
Evaporation
Fibroblasts
Fluorescence
Fluorescence microscopy
Hypothyroidism
Immune response
Infrared analysis
Iodide peroxidase
Leaching
Lymphocytes
Lymphocytes T
Medical treatment
Nanoparticles
NMR
Nuclear magnetic resonance
Oxidative stress
Peroxidase
Polyethylene glycol
Polymer blends
Polyurethane
Polyurethane resins
Selenium
Thyroid
Thyroid gland
thyroid hormones
Vinyl acetate
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Summary:Hypothyroidism is an autoimmune disease associated with underactive thyroid gland. In this study, a dual effect polymeric system was designed to release Cepharanthine (CEP) to block T cell activation and Selenium (Se) to decrease the anti‐thyroid peroxidase (TPOAb) concentration in order to treat hypothyroidism. For this purpose, poly(ethylene‐vinyl acetate) (PEVA) and polyethylene glycol (PEG) nanoparticles (NPs) including CEP were synthesized by emulsion solvent evaporation method and they were loaded to polyurethane (PU)/PEG‐PUSe‐PEG block copolymer blends which were fabricated by particulate leaching technique as porous sponges. Fourier‐Transform Infrared (FTIR), Raman, and Nuclear Magnetic Resonance (NMR) analysis showed successful synthesis of PEG‐PUSe‐PEG block copolymer. A long‐term zero‐order release profile was obtained for CEP. Se release rate from matrices showed an oxidative stress‐mediated release which can be used to adjust Se amount. According to MTS results conducted by NIH 3T3 fibroblasts, both NPs and matrices have no adverse effect on cell viability. Fluorescence microscopy and SEM images confirm the MTS results. The dual release system has potential to be effectively used in long‐term treatment of hypothyroidism by addressing both auto‐immune response and hormone regulation.
ISSN:1549-3296
1552-4965
DOI:10.1002/jbm.a.36855