Synaptotagmin 1 regulates cortical granule exocytosis during mouse oocyte activation

Synaptotagmin 1 (Syt1) is an abundant and important presynaptic vesicle protein that binds Ca2+ for the regulation of synaptic vesicle exocytosis. Our previous study reported its localization and function on spindle assembly in mouse oocyte meiotic maturation. The present study was designed to inves...

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Published in:Zygote (Cambridge) 2020-04, Vol.28 (2), p.97-102
Main Authors: Zhu, Xiu-Lan, Li, Shi-Fen, Zhang, Xi-Qian, Xu, Hong, Luo, Yan-Qun, Yi, Yan-Hong, Lv, Li-Juan, Zhang, Chun-Hui, Wang, Zhen-Bo, Ouyang, Ying-Chun, Hou, Yi, Schatten, Heide, Liu, Feng-Hua
Format: Article
Language:English
Subjects:
Actin
Activation
Animals
Calcium (intracellular)
Calcium - metabolism
Calcium ions
Calcium signalling
Cell cycle
Confocal microscopy
Eggs
Exocytosis
Female
Gametocytes
Granular materials
Kinases
Localization
Meiosis
Mice
Neuroimaging
Oocytes - metabolism
Oogenesis
Proteins
Roles
Signal generation
Software
Sperm
Synaptotagmin
Synaptotagmin I - genetics
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cited_by cdi_FETCH-LOGICAL-c372t-9d20424559252f3051fdc87e916fe9c3e6bd7969079e14b235499110f1eb41633
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container_issue 2
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container_title Zygote (Cambridge)
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creator Zhu, Xiu-Lan
Li, Shi-Fen
Zhang, Xi-Qian
Xu, Hong
Luo, Yan-Qun
Yi, Yan-Hong
Lv, Li-Juan
Zhang, Chun-Hui
Wang, Zhen-Bo
Ouyang, Ying-Chun
Hou, Yi
Schatten, Heide
Liu, Feng-Hua
description Synaptotagmin 1 (Syt1) is an abundant and important presynaptic vesicle protein that binds Ca2+ for the regulation of synaptic vesicle exocytosis. Our previous study reported its localization and function on spindle assembly in mouse oocyte meiotic maturation. The present study was designed to investigate the function of Syt1 during mouse oocyte activation and subsequent cortical granule exocytosis (CGE) using confocal microscopy, morpholinol-based knockdown and time-lapse live cell imaging. By employing live cell imaging, we first studied the dynamic process of CGE and calculated the time interval between [Ca2+]i rise and CGE after oocyte activation. We further showed that Syt1 was co-localized to cortical granules (CGs) at the oocyte cortex. After oocyte activation with SrCl2, the Syt1 distribution pattern was altered significantly, similar to the changes seen for the CGs. Knockdown of Syt1 inhibited [Ca2+]i oscillations, disrupted the F-actin distribution pattern and delayed the time of cortical reaction. In summary, as a synaptic vesicle protein and calcium sensor for exocytosis, Syt1 acts as an essential regulator in mouse oocyte activation events including the generation of Ca2+ signals and CGE.
doi_str_mv 10.1017/S0967199419000704
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source Cambridge University Press journals
subjects Actin
Activation
Animals
Calcium (intracellular)
Calcium - metabolism
Calcium ions
Calcium signalling
Cell cycle
Confocal microscopy
Eggs
Exocytosis
Female
Gametocytes
Granular materials
Kinases
Localization
Meiosis
Mice
Neuroimaging
Oocytes - metabolism
Oogenesis
Proteins
Roles
Signal generation
Software
Sperm
Synaptotagmin
Synaptotagmin I - genetics
title Synaptotagmin 1 regulates cortical granule exocytosis during mouse oocyte activation
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