Acinetobacter pullicarnis sp. nov. isolated from chicken meat

• Published in: Archives of microbiology 2020-05, Vol.202 (4), p.727-732
• Main Authors: Han, Rae-Hee, Lee, Ju-Eun, Yoon, Sung-Hee, Kim, Geun-Bae
• Format: Article
• Language: English
• Subjects: Acinetobacter; Acinetobacter - classification; Acinetobacter - genetics; Acinetobacter - isolation & purification; Animals; Base Composition; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cardiolipin; Catalase; Cell Biology; Chickens; Chickens - microbiology; Deoxyribonucleic acid; DNA; DNA, Bacterial - genetics; Ecology; Fatty acids; Fatty Acids - analysis; Hybridization; Life Sciences; Lipids; Meat; Meat - microbiology; Microbial Ecology; Microbiology; New species; Nucleic Acid Hybridization; Nucleotides; Original Paper; pH effects; Phosphatidylglycerol; Phosphatidylserine; Phylogeny; Quinones; Quinones - analysis; Republic of Korea; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence analysis; Sequence Analysis, DNA
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-019-01785-y

A novel bacterial strain, named S23 T , was isolated from chicken meat of local market in Korea. Cells were Gram-negative, milky-yellow colored, non-motile and coccobacillus. The strain was obligate aerobic and catalase-positive, oxidase-negative, optimum growth temperature and pH were 25 °C and pH 7.0, respectively. On the basis of 16S rRNA gene sequence analysis, strain S23 T belongs to the genus Acinetobacter and is most closely related to Acinetobacter defluvii KCTC 52503  T (97.40%). The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) value between strain S23 T and its closet phylogenetic neighbors was below 76% and 17%, respectively. The G + C content of genomic DNA of strain S23 T was 41.53 mol%. The major respiratory quinone was Q-9. The major cellular fatty acids were summed feature 3 (comprising C 16:1 ω 7 c and/or C 16:1 ω 6 c ), C 18:1 ω 9 c , and C 16:0 . The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanol-amine, and phosphatidylserine. The ANI and dDDH results and results of the genotypic analysis in combination with chemotaxonomic and physiological data demonstrated that strain S23 T represented a novel species within the genus Acinetobacter , for which the name Acinetobacter pullicarnis sp. nov. is proposed. The strain type is S23 T (= KACC 19921  T  = JCM 33150  T ).