The Combined Usage of β-Cyclodextrin and Milk Proteins in Microencapsulation of Bifidobacterium bifidum BB-12

• Published in: Probiotics and antimicrobial proteins 2020-06, Vol.12 (2), p.747-755
• Main Author: Arslan-Tontul, Sultan
• Format: Article
• Language: English
• Subjects: Applied Microbiology; Bifidobacterium bifidum; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Drying; Intestine; Microbiology; Microcapsules; Microencapsulation; Microparticles; Nutrition; Oxidation; Protein Science; Proteins; Whey protein; β-Cyclodextrin
• ISSN: 1867-1306; 1867-1314
• DOI: 10.1007/s12602-019-09621-x

The present study aimed to determine the effects of combined usage of β-cyclodextrin with whey protein isolate and sodium caseinate on the microencapsulation of Bifidobacterium bifidum- BB12 by spray drying. From the results, the highest count of B. bifidum was provided by whey protein isolate as 8.62 log CFU/g. The increasing concentration of β-cyclodextrin considerably increases gastric and intestinal resistance to B. bifidum cells. In the gastric and intestinal test, the highest protection was determined in whey protein isolate substituted with 10% β-cyclodextrin with reduction rates of 0.98 and 3.30%, respectively. Moreover, free cells did not survive in the same gastric conditions. The lowest hygroscopicity was determined in whey protein isolate as 8.57%. It must be noted that increasing β-cyclodextrin concentration in carrier material combination led to an increase in hygroscopicity of microcapsules. In general, substitution with β-cyclodextrin increased the particle size of microparticles, and microcapsules produced with whey protein isolate had a smaller size than that of sodium caseinate.