Amycolatopsis alkalitolerans sp. nov., isolated from Gastrodia elata Blume

• Published in: Journal of antibiotics 2020-01, Vol.73 (1), p.35-39
• Main Authors: Narsing Rao, Manik Prabhu, Li, Yan-Qiong, Zhang, Hui, Dong, Zhou-Yan, Dhulappa, Awalagaway, Xiao, Min, Li, Wen-Jun
• Format: Article
• Language: English
• Subjects: 631/326; 631/326/41; 96/63; Actinomycetales - chemistry; Actinomycetales - classification; Actinomycetales - isolation & purification; Bacteriology; Base Composition; Base Sequence; Biomedical and Life Sciences; Bioorganic Chemistry; Fatty Acids - chemistry; Gastrodia - microbiology; Genes, Bacterial; Life Sciences; Lipids; Lipids - chemistry; Medicinal Chemistry; Microbiology; Organic Chemistry; Peptidoglycan - chemistry; Phospholipids - chemistry; Plant Tubers - microbiology; RNA, Ribosomal, 16S - analysis; Sodium chloride; Sugars - chemistry; Vitamin K 2 - chemistry
• ISSN: 0021-8820; 1881-1469
• DOI: 10.1038/s41429-019-0222-8

A Gram-staining positive and nonmotile strain designated SYSUP0005 T was isolated from tubers of Gastrodia elata Blume. The 16S rRNA gene sequence result showed that strain SYSUP0005 T shared highest sequence similarity with the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%). Growth occurs at 14–37 °C (optimum temperature, 28 °C), at pH 6–9 (optimum, pH 8) and in the presence of up to 6% (w/v) NaCl. Strain SYSUP0005 T had meso -diaminopimelic acid in its peptidoglycan. The whole cell sugars were galactose, ribose, and xylose. The predominant menaquinone was MK-9(H 4 ) and minor menaquinones were MK-9(H 2 ) and MK-9(H 8 ). The polar lipids were diphosphatidylglycerol (DPG); phosphatidylmonomethylethanolamine (PME), phosphatidylethanolamine (PE), phosphatidylinositol (PI), unidentified glycolipid (GL), and unidentified phospholipid (PL). The genomic DNA G + C content was 69.6 mol%. The major fatty acids were iso-C 16:0 , anteiso-C 17:0 , C 16:0 , iso-C 14:0 , C 17:1 ω 6 c , C 17:0 , and Summed Feature 3 (C 16:1 ω 7 c /C 16:1 ω 6 c ). On the basis of the phenotypic, phylogenetic, chemotaxonomic characters, and genomic comparison, SYSUP0005 T represents a novel species of the genus Amycolatopsis , for which the name Amycolatopsis alkalitolerans sp. nov. is proposed. The type strain is SYSUP0005 T (=KCTC 49024 T  = CGMCC4.7463 T ).