Computerized Calculation of Mitotic Count Distribution in Canine Cutaneous Mast Cell Tumor Sections: Mitotic Count Is Area Dependent

• Published in: Veterinary pathology 2020-03, Vol.57 (2), p.214-226
• Main Authors: Bertram, Christof A., Aubreville, Marc, Gurtner, Corinne, Bartel, Alexander, Corner, Sarah M., Dettwiler, Martina, Kershaw, Olivia, Noland, Erica L., Schmidt, Anja, Sledge, Dodd G., Smedley, Rebecca C., Thaiwong, Tuddow, Kiupel, Matti, Maier, Andreas, Klopfleisch, Robert
• Format: Article
• Language: English
• Subjects: Animals; Cell Count - veterinary; Dog Diseases - pathology; Dogs; Histological Techniques - veterinary; Image Processing, Computer-Assisted; Mast Cells - pathology; Mitotic Index - veterinary; Neoplasm Grading - veterinary; Observer Variation; Pathologists; Skin Neoplasms - pathology; Skin Neoplasms - veterinary; Software
• ISSN: 0300-9858; 1544-2217
• DOI: 10.1177/0300985819890686

Mitotic count (MC) is an important element for grading canine cutaneous mast cell tumors (ccMCTs) and is determined in 10 consecutive high-power fields with the highest mitotic activity. However, there is variability in area selection between pathologists. In this study, the MC distribution and the effect of area selection on the MC were analyzed in ccMCTs. Two pathologists independently annotated all mitotic figures in whole-slide images of 28 ccMCTs (ground truth). Automated image analysis was used to examine the ground truth distribution of the MC throughout the tumor section area, which was compared with the manual MCs of 11 pathologists. Computerized analysis demonstrated high variability of the MC within different tumor areas. There were 6 MCTs with consistently low MCs (MC