Pararcticibacter amylolyticus gen. nov., sp. nov., Isolated from a Rotten Hemp Rope, and Reclassification of Pedobacter tournemirensis as Pararcticibacter tournemirensis comb. nov

• Published in: Current microbiology 2020-02, Vol.77 (2), p.320-326
• Main Authors: Cai, Yu-yuan, Dong, Wei-wei, Hu, Yuan-liang, Jiang, Xu, Wang, Yan-wei, Zhou, Yi-qing, Peng, Nan, Liang, Yun-xiang, Zhao, Shu-miao
• Format: Article
• Language: English
• Subjects: Bacterial Typing Techniques; Bacteroidetes - classification; Bacteroidetes - isolation & purification; Base Composition; Biomedical and Life Sciences; Biotechnology; Cannabis - microbiology; China; Deoxyribonucleic acid; DNA; DNA, Bacterial - genetics; Fatty acids; Fatty Acids - chemistry; Gene sequencing; Genera; Genomes; Hemp; Hybridization; Life Sciences; Lipids; Microbiology; New species; Nucleotide sequence; Pedobacter - classification; Phosphatidylethanolamine; Phospholipids - chemistry; Phylogenetics; Phylogeny; Polyamines; Quinones; Reclassification; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA; Species classification; Taxonomic revision; Vitamin K 2 - analogs & derivatives; Vitamin K 2 - chemistry
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-019-01828-z

A Gram-stain-negative, rod-shaped, non-motile, facultatively anaerobic bacterium, designated FJ4-8 T , was isolated from a rotten hemp rope in Chongqing City, PR China. Phylogenetic analysis of 16S rRNA gene sequences indicated that the isolate was closely related to members of the family Sphingobacteriaceae , with the highest similarity to Pedobacter tournemirensis TF5-37.2-LB10 T (95.3%) and low similarities to all other species of the genus Pedobacter (90.4–93.9%). Phylogenetic analyses demonstrated that strain FJ4-8 T formed a stable subclade with Pedobacter tournemirensis TF5-37.2-LB10 T . The clade with these two strains branched adjacent to a clade containing three species of the genus Arcticibacter . MK-7 was detected as the only respiratory quinone. The major fatty acids composed iso-C 15:0 , iso-C 17:0 3-OH and summed feature three. Phosphatidylethanolamine, three aminophospholipids and one unidentified lipid were found as the major polar lipids. The major polyamine was identified as sym -homospermidine. The DNA–DNA hybridization value between strain FJ4-8 T and Pedobacter tournemirensis TF5-37.2-LB10 T was 42.0 ± 2.5%. Based on its phylogenetic, chemotaxonomic and phenotypic characteristics, the novel strain and TF5-37.2-LB10 T were found to be different from members of genera Pedobacter and Arcticibacter . FJ4-8 T and TF5-37.2-LB10 T represented different species. Therefore, FJ4-8 T should be classified as a novel species of a novel genus in the family Sphingobacteriaceae , for which the name Pararcticibacter amylolyticus gen. nov., sp. nov. is proposed. The type strain is FJ4-8 T (= KCTC 62640 T  = CCTCC AB 2018052 T ). The draft genome sequence is 6290, 449 bp in length, the genomic DNA G+C content was 44.4 mol%. Pedobacter tournemirensis TF5-37.2-LB10 T should be transferred to the novel genus as Pararcticibacter tournemirensis comb. nov. (The type strain is TF5-37.2-LB10 T (= DSM 23085 T  = CIP 110085 T  = MOLA 820 T ).