Structural evolution of supported lipid bilayers intercalated with quantum dots

[Display omitted] Supported lipid bilayers (SLBs) embedded with hydrophobic quantum dots (QDs) undergo temporal structural rearrangement. Synchrotron X-ray reflectivity (XRR) was applied to monitor the temporal structural changes over a period of 24 h of mixed SLBs of 1-palmitoyl-2-oleoyl-sn-glycero...

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Bibliographic Details
Published in:Journal of colloid and interface science 2020-03, Vol.562, p.409-417
Main Authors: Wlodek, Magdalena, Slastanova, Anna, Fox, Laura J., Taylor, Nicholas, Bikondoa, Oier, Szuwarzynski, Michal, Kolasinska-Sojka, Marta, Warszynski, Piotr, Briscoe, Wuge H.
Format: Article
Language:English
Subjects:
Bilayer structure
Membranes intercalated with quantum dots
Nanoparticle-membrane interactions
Supported lipid bilayers
X-ray reflectivity (XRR)
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Summary:[Display omitted] Supported lipid bilayers (SLBs) embedded with hydrophobic quantum dots (QDs) undergo temporal structural rearrangement. Synchrotron X-ray reflectivity (XRR) was applied to monitor the temporal structural changes over a period of 24 h of mixed SLBs of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) / 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-ethanolamine (POPE) intercalated with 4.9 nm hydrophobic cadmium sulphide quantum dots (CdS QDs). The QD-embedded SLBs (QD-SLBs) were formed via rupture of the mixed liposomes on a positively charged polyethylene imine (PEI) monolayer. Atomic force microscopy (AFM) imaging provided complementary characterization of the bilayer morphology. Our results show time-dependent perturbations in the SLB structure due to the interaction upon QD incorporation. Compared to the SLB without QDs, at 3 h incubation time, there was a measurable decrease in the bilayer thickness and a concurrent increase in the scattering length density (SLD) of the QD-SLB. The QD-SLB then became progressively thicker with increasing incubation time, which – along with the fitted SLD profile – was attributed to the structural rearrangement due to the QDs being expelled from the inner leaflet to the outer leaflet of the bilayer. Our results give unprecedented mechanistic insights into the structural evolution of QD-SLBs on a polymer cushion, important to their potential biomedical and biosensing applications.
ISSN:0021-9797
1095-7103
DOI:10.1016/j.jcis.2019.11.102