Production of distinct labdane-type diterpenoids using a novel cryptic labdane-like cluster from Streptomyces thermocarboxydus K155

Bioinformatic mining of the Streptomyces thermocarboxydus K155 genome predicted the presence of four synthases for the production of geosmin, hopene, albaflavenone, and a type B-type A diterpenoid system like that described for labdane-related diterpenoids (LRD). The lrd cluster was comprised by an...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2020-01, Vol.104 (2), p.741-750
Main Authors: Guzmán-Trampe, Silvia M., Ikeda, Haruo, Vinuesa, Pablo, Macías-Rubalcava, Martha L., Esquivel, Baldomero, Centeno-Leija, Sara, Tapia-Cabrera, Silvana M., Mora-Herrera, Silvia I., Ruiz-Villafán, Beatriz, Rodríguez-Sanoja, Romina, Sanchez, Sergio
Format: Article
Language:English
Subjects:
Analysis
Biomedical and Life Sciences
Biotechnology
Chromosomes
Cloning
Cytochrome
Diterpenes
E coli
Enzymes
Genes
Genomes
Genomics
Geosmin
Life Sciences
Methicillin
Microbial Genetics and Genomics
Microbiology
Mycelia
Neomycin
Plasmids
Proteomics
Staphylococcus infections
Streptomyces
Streptomyces coelicolor
Ticarcillin
Transcriptomics
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Summary:Bioinformatic mining of the Streptomyces thermocarboxydus K155 genome predicted the presence of four synthases for the production of geosmin, hopene, albaflavenone, and a type B-type A diterpenoid system like that described for labdane-related diterpenoids (LRD). The lrd cluster was comprised by an operon of four genes ( lrdABDC ). This cluster seemed to be silent in the wild-type strain, as neither labdane nor terpene-like compounds were detected by UPLC-TOF-MS and GC-MS analyses in both culture supernatants and mycelial extracts. Heterologous expression of the lrdABDC cluster in a defective cyslabdan producer ( Streptomyces cyslabdanicus K04-0144Δ cld ) generated 8,17-epoxy-7-hydroxy labda-12,14-diene and cyslabdan. The same cluster expressed in the strains Streptomyces coelicolor M1152, Streptomyces peucetius var. caesius , and Streptomyces avermitilis SUKA22 produced the general intermediary labda-8(17), 12(E),14-triene [( E )-biformene]. Besides ( E )-biformene, S. coelicolor M1152 and S. avermitilis SUKA22 produced two and three different labdane-type diterpenoids, underlying the relevance of the genetic background of the Streptomyces host in product formation.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-019-10240-3