Effect of Black Grape Seed Extract (Vitis vinifera) on Biofilm Formation of Methicillin-Resistant Staphylococcus aureus and Staphylococcus haemolyticus

Grape seeds are considered one of the most important sources for phenolic and other compounds and is globally consumed for the biological value of its active ingredients. The increasing prevalence of Methicillin-resistant Staphylococcus aureus -related infections has become a very challenging health...

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Bibliographic Details
Published in:Current microbiology 2020-02, Vol.77 (2), p.238-245
Main Authors: Al-Mousawi, Alia Hussein, Al-kaabi, Siham Jasim, Albaghdadi, Ahmad J. H., Almulla, Abbas F., Raheem, Ameer, Algon, Ali Abbas Abo
Format: Article
Language:English
Subjects:
Antibacterial activity
Antibiotics
Bacteria
Biofilms
Biomedical and Life Sciences
Biotechnology
Catechin
Drug resistance
Esters
Fruits
Gallic acid
Grapes
Laboratory equipment
Life Sciences
Methicillin
Microbiology
Minimum inhibitory concentration
Moxifloxacin
Penicillin
Phenolic compounds
Phenols
Plant extracts
Seeds
Staphylococcus
Staphylococcus aureus
Staphylococcus haemolyticus
Staphylococcus infections
Synergistic effect
Virulence
Vitis vinifera
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Summary:Grape seeds are considered one of the most important sources for phenolic and other compounds and is globally consumed for the biological value of its active ingredients. The increasing prevalence of Methicillin-resistant Staphylococcus aureus -related infections has become a very challenging health issue worldwide. This work aims at examining the antibacterial activity of alcoholic extract of black grape seeds ( Vitis vinifera ) against biofilm formation by Staphylococcus aureus and Staphylococcus haemolyticus . Staphylococcal bacterial isolates were first clinically confirmed using the VITEK-2compact system (ID and AST), and four isolates were selected depending on virulence and resistance to different types of antibiotics. The ability of S. aureus and S. haemolyticus isolates to form biofilm was examined using a standardized 96-well microtiter plate method. Furthermore, the effect of Moxifloxacin and Penicillin G with MIC, sub-MIC and sub-sub-MIC in preventing S. aureus and S. haemolyticus biofilm production, as well as that of the grape seed extract (180 mg/ml) were tested against biofilm formation. Our data indicate that all of the Staphylococcal bacterial isolates were able to produce biofilm which was prevented by the methanolic extracts of the crude seeds of Vitis vinifera rich in galloylated catechin esters of gallic acid. A significant ( P  
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-019-01827-0