Molecular characterization and function analysis of Epinephelus coioides Hsp22 response to SGIV and Vribro alginolyticus infection

Heat shock protein 22 (Hsp22) is an important regulatory factor response to various stresses in mammals. In this study, the full length cDNA of Epinephelus coioides Hsp22, which was 1680bp in length, with a 289 bp 5′ UTR, a 725 bp 3′UTR, and a 666 bp open reading frame encoding 221 amino acids, was...

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Published in:Fish & shellfish immunology 2020-02, Vol.97, p.125-134
Main Authors: Li, Pin-Hong, Liang, Yu-Lin, Su, Yu-Ling, Jiang, Yu-Feng, Chen, Biao, Chen, Shu-Yin, Huang, You-Hua, Wei, Jing-Guang, Huang, Xiao-Hong, Qin, Qi-Wei, Sun, Hong-Yan
Format: Article
Language:English
Subjects:
Apoptosis
Epinephelus coioides
Hsp22
SGIV
Vibrio alginolyticus
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Summary:Heat shock protein 22 (Hsp22) is an important regulatory factor response to various stresses in mammals. In this study, the full length cDNA of Epinephelus coioides Hsp22, which was 1680bp in length, with a 289 bp 5′ UTR, a 725 bp 3′UTR, and a 666 bp open reading frame encoding 221 amino acids, was obtained. E. coioides Hsp22 contains a highly conserved α-crystallin domain. E. coioides Hsp22 mRNA was detected in all tissues examined by quantitative real-time PCR, with the highest expression in blood, followed by the spleen, skin, gill, head kidney, muscle, heart, liver, trunk kidney, stomach, pyloric caeca, intestine, brain and thymus. The expression patterns of E. coioides Hsp22 response to infection with Singapore grouper iridovirus (SGIV) and Vribro alginolyticus, the important pathogens of E. coioides, were studied. The expression levels of the gene were up-regulated in the tissues examined. Subcellular localization analysis demonstrated that E. coioides Hsp22 was distributed in both the cytoplasm and nucleus. In addition, E. coioides Hsp22 significantly inhibited the SGIV-induced cell apoptosis. In summary, the E. coioides Hsp22 might play a critical role in pathogenic stimulation. •Hsp22 gene was identified from Epinephelus coioides.•Hsp22 of E. coioides was distributed throughout the cell.•Hsp22 of E. coioides in tissues examined was significantly up-regulated response to Vibrio alginolyticus and SGIV infection.•coioides Hsp22 significantly inhibited SGIV-induced apoptosis.
ISSN:1050-4648
1095-9947
DOI:10.1016/j.fsi.2019.11.069