Assessment of Aedes aegypti (Diptera: Culicidae) Males Flight Ability for SIT Application: Effect of Device Design, Duration of Test, and Male Age

• Published in: Journal of medical entomology 2020-05, Vol.57 (3), p.824-829
• Main Authors: Dor, Ariane, Maggiani-Aguilera, Ana María, Valle-Mora, Javier, Bond, J. Guillermo, Marina, Carlos F., Liedo, Pablo
• Format: Article
• Language: English
• Subjects: Aedes - physiology; Aedes aegypti; Age Factors; Animals; Aquatic insects; Control methods; Devices; Flight; Flight tests; Flight, Animal; Insects; integrated vector management; Male; Male sterility; Males; mass rearing; Mosquito Control - instrumentation; Mosquito Control - methods; Mosquitoes; Pest control; Pest Control, Biological - instrumentation; Pest Control, Biological - methods; Quality control; Reproduction; Sexual Behavior, Animal; Sterile Insect Technique; Sterility; Sterilized organisms; Testing time; VECTOR CONTROL, PEST MANAGEMENT, RESISTANCE, REPELLENTS; Vertical flight
• ISSN: 0022-2585; 1938-2928
• DOI: 10.1093/jme/tjz226

The Sterile InsectTechnique (SIT) is a pest control method where large numbers of sterile males are released to induce sterility in wild populations. Since a successful SIT application depends on the released sterile males being competitive with wild males, standard quality control tests are a necessary component of any SIT program. Flight ability (ability to fly out from a device) is a reliable indicator of insect quality. Based on previous studies, we developed four new tubular devices constructed with locally available materials to explore their potential as flight test devices for Aedes aegypti (L.) mass-reared males. Males were allowed to fly upwards through a vertical tube, the ones that flew out were considered successful. The effect of male age (0 to 21 d old), test time interval (30 min to 24 h), and the design of the device (40 and 80 cm height and 2 and 3.5 cm diameter) were evaluated. Our devices determined differences in the flight ability of Ae. aegypti males of different ages. During the first minutes, more old males escaped than young males in three out of four types of devices. However, young males reached higher rates of escape in all cases after 24 h. For standard quality control tests, we recommend testing 2- to 3-d-old sexually mature males in the high and narrow device (80 × 2 cm). Further observations for time intervals between 1 and 5 h might be performed to decide the shortest and more representative interval to use.