Human skin fibrosis: up‐regulation of collagen type III gene transcription in the fibrotic skin nodules of lower limb lymphoedema

Objectives To investigate the cellular and molecular pathophysiology involved in the development of fibrotic skin of grade‐3 lymphoedema patients with a focus on collagen types. Methods Fibrotic and normal skin biopsy samples obtained from grade‐3 lymphoedema patients and normal individuals, respect...

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Bibliographic Details
Published in:Tropical medicine & international health 2020-03, Vol.25 (3), p.319-327
Main Authors: Karayi, Arun Kumar, Basavaraj, Vijaya, Narahari, Saravu R., Aggithaya, Madhur Guruprasad, Ryan, Terence J., Pilankatta, Rajendra
Format: Article
Language:English
Subjects:
analyse de transcription génétique
Biopsy
Collagen
Collagen (type I)
Collagen (type III)
connective tissue disorders
Dermis
fibrose
Fibrosis
filariose lymphatique
Gene expression
gene transcript analysis
Histopathology
Image enhancement
Immunohistochemistry
integrative medicine
lymphatic filariasis
Lymphedema
lymphoedema
lymphœdème
médecine intégrative
Nodules
Skin
Transcription
troubles du tissu conjonctif
Up-regulation
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Summary:Objectives To investigate the cellular and molecular pathophysiology involved in the development of fibrotic skin of grade‐3 lymphoedema patients with a focus on collagen types. Methods Fibrotic and normal skin biopsy samples obtained from grade‐3 lymphoedema patients and normal individuals, respectively, were analysed by histopathology, quantitative real‐time PCR and immunohistochemistry to examine collagen gene expression. Results Histopathologic analysis revealed epidermal changes such as orthokeratosis, hypergranulosis and irregular acanthosis in the skin biopsies. The thickened dermis contained nodules of haphazardly arranged thick collagen bundles. Real‐time PCR data showed significant (P‐value 0.0003) up‐regulation of Collagen type I and type III gene transcripts in the fibrotic skin of patients resulting in 38.94‐fold higher transcription of Collagen type III alpha‐1 gene than of Collagen type I alpha‐1 gene. Semi‐quantification of the per cent of haematoxylin‐DAB‐stained area of immunohistochemistry images also showed significant (P 
ISSN:1360-2276
1365-3156
DOI:10.1111/tmi.13359