PepA binds to and negatively regulates esrB to control virulence in the fish pathogen Edwardsiella piscicida

As an important marine fish pathogen, Edwardsiella piscicida infects a broad range of fish species and causes substantial economic losses. The EsrA-EsrB two-component system is essential for the expression of type III and type VI secretion systems (T3/T6SSs), the key virulence determinants in the ba...

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Bibliographic Details
Published in:Microbiological research 2020-02, Vol.232, p.126349-126349, Article 126349
Main Authors: Yin, Kaiyu, Peng, Yue, Ahmed, Moamer A.H., Ma, Jiabao, Xu, Rongjing, Zhang, Yuanxing, Ma, Yue, Wang, Qiyao
Format: Article
Language:English
Subjects:
Animals
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Edwardsiella - genetics
Edwardsiella - metabolism
Edwardsiella piscicida
Electrophoretic Mobility Shift Assay - veterinary
Enterobacteriaceae Infections - microbiology
Enterobacteriaceae Infections - veterinary
EsrB
Fish Diseases - microbiology
Gene Expression Regulation, Bacterial
In vivo colonization
Leucyl aminopeptidase
Leucyl Aminopeptidase - genetics
Leucyl Aminopeptidase - metabolism
Promoter Regions, Genetic
Pull-down
Reactive Oxygen Species
Type III Secretion Systems - metabolism
Type VI Secretion Systems - metabolism
Virulence - genetics
Virulence Factors - genetics
Virulence Factors - metabolism
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Summary:As an important marine fish pathogen, Edwardsiella piscicida infects a broad range of fish species and causes substantial economic losses. The EsrA-EsrB two-component system is essential for the expression of type III and type VI secretion systems (T3/T6SSs), the key virulence determinants in the bacterium. In this study, a pull-down assay with the esrB promoter as bait was performed to identify the upstream regulators of esrB. As a result, PepA, a leucyl aminopeptidase, was identified as a repressor of EsrB and T3/T6SS expression. PepA bound to the esrB promoter region and negatively regulated the production of T3/T6SS proteins in early stages. Moreover, PepA was found to affect the in vivo colonization of E. piscicida in turbot livers through the regulation of EsrB expression. Collectively, our results enhance the understanding of the virulence regulatory network and in vivo colonization mechanism of E. piscicida. One sentence summary: PepA regulates EsrB expression in Edwardsiella piscicida.
ISSN:0944-5013
1618-0623
DOI:10.1016/j.micres.2019.126349